Glass capillary tube feeding:: A method for infecting nymphal Ixodes scapularis (Acari: Ixodidae) with the Lyme disease spirochete Borrelia burgdorferi
Glass capillary tube feeding:: A method for infecting nymphal Ixodes scapularis (Acari: Ixodidae) with the Lyme disease spirochete Borrelia burgdorferi
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DOI：
10.1603/0022-2585-39.2.285
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发表时间：
2002-03-01
影响因子：
2.1
通讯作者：
De Silva, AM
中科院分区：
文献类型：
--
作者：
Broadwater, AH;Sonenshine, DE;De Silva, AM
研究了人工毛细血管喂养法感染莱姆病病原伯氏疏螺旋体（Borrelia burgdorferi）。30% ~ 70%的若虫从充满螺旋体溶液的玻璃毛细管中摄食2.5 h后感染。摄食后，若虫的毛细血管感染稳定且持续至少10 d。毛细血管摄食也保持了自然媒介能力模式，因为与不受感染的变皮蜱不同，镰形蜱获得了感染。毛细感染的肩胛棘球蚴若虫能够将感染传染给幼鼠，但不如自然感染的若虫有效。毛细管感染方法简便，是一种较好的替代注射接种方法感染动物伯氏疏螺旋体。
We evaluated an artificial capillary feeding method to infect nymphal Ixodes scapularis (Say) ticks with Borrelia burgdorferi, the causative agent of Lyme disease. Thirty to 70% of the nymphs were infected after feeding for 2.5 h from glass capillary tubes filled with a solution of spirochetes. Capillary infection was stable and persisted in the nymphs for at least 10 d after feeding. Capillary feeding also maintained natural vector competence patterns because I. scapularis ticks acquired infection unlike Dermacentor variablis (Say), which did not become infected. Capillary infected I. scapularis nymphs were capable of transmitting the infection to naive mice although not as efficiently as naturally infected nymphs. The capillary infection method is convenient and is a better alternative to syringe inoculation as a means of infecting animals with B. burgdorferi.