Genetic Diversity of Campylobacter jejuni Isolated From Avian and Human Sources in Egypt
Genetic Diversity of Campylobacter jejuni Isolated From Avian and Human Sources in Egypt
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DOI：
10.3389/fmicb.2019.02353
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发表时间：
2019-10-18
影响因子：
5.2
通讯作者：
Bendary, Mahmoud M.
中科院分区：
文献类型：
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作者：
Abd El-Hamid, Marwa, I;Abd El-Aziz, Norhan K.;Bendary, Mahmoud M.
空肠弯曲菌（C.空肠）能够定殖和感染家禽，也对人类构成风险。本研究的目的是确定C.从埃及的禽类和人类来源回收的空肠分离株。此外，鞭毛蛋白A（flaA）基因的短可变区（SVR）的等位变异的存在进行了分析。结果表明，C.空肠分离株在它们单独或以各种组合存在时携带每种毒力基因的能力上不同。flaA基因在所有C. jejuni菌株，没有一个菌株具有所有研究的毒力基因。当考虑C. jejuni菌株中，cdtC基因最相似，而cdtB和iam基因最不相似。我们可以确定13个新的等位基因的分析菌株。对毒力基因型、flaA基因序列和等位变异的分析表明，C.不同来源的空肠弯曲菌菌株在很大程度上重叠，表明家禽可能参与传播C.空肠移植给人类我们还发现，从同一宿主分离的菌株是高度异质性的，鸡株表现出最高的多样性。此外，人类菌株更接近鸡而不是鸽子。本研究的结果应考虑到在评估埃及C的流行病学和风险潜力。空肠不仅在家禽中，而且在人类中。
Campylobacter jejuni (C. jejuni) are able to colonise and infect domestic poultry and also pose a risk for humans. The aim of this study was to determine the extent of genotypic diversity among C. jejuni isolates recovered from avian and human sources in Egypt. Furthermore, the short variable region (SVR) of flagellin A (flaA) gene was analysed for the presence of allelic variants. Our results showed that C. jejuni isolates differ in their capacity to harbour each of the virulence genes alone or when present in various combinations. The flaA gene was detected in all C. jejuni strains and none of the strains had all the studied virulence genes together. When considering C. jejuni strains from the investigated sources, the cdtC gene was the most similar, while the cdtB and iam genes were the most dissimilar. We could identify 13 novel alleles in the analysed strains. The analyses of virulence gene patterns, flaA gene sequences and allelic variants showed that C. jejuni strains from different sources overlapped largely suggesting potential involvement of poultry in transmitting C. jejuni to humans. We also found that the strains isolated from the same host were highly heterogeneous, with chicken strains exhibiting the highest diversity. Moreover, the human strains were clustered closer to chicken ones than to those from pigeon. The results of this study should be taken into consideration when assessing the epidemiology and risk potential of Egyptian C. jejuni not only in poultry, but also in humans.