BEHAVIORAL-STUDIES FOLLOWING LESIONS OF MESOLIMBIC AND MESOSTRIATAL SEROTONERGIC PATHWAYS
BEHAVIORAL-STUDIES FOLLOWING LESIONS OF MESOLIMBIC AND MESOSTRIATAL SEROTONERGIC PATHWAYS
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DOI：
10.1016/0006-8993(76)91024-6
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
MANDELL, AJ
中科院分区：
文献类型：
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作者：
GEYER, MA;PUERTO, A;MANDELL, AJ
对选择性损毁中缝背核(B7)、中缝正中核(B8)或中缝外侧核(B9)的大鼠在不同实验条件下的行为进行了比较。用荧光组织化学法测定大鼠中脑中缝核团的损伤程度，并测定大鼠前脑6个区域的色氨酸羟基酶和酪氨酸羟基酶活性。所有病变均未影响酪氨酸羟基酶活性。B7的损伤降低了纹状体、丘脑、皮质和下丘脑中的色氨酸羟化酶，对任何行为指标都没有显著影响。B9的损伤，尽管大小是B9的两倍，但既没有显著降低前脑色氨酸羟基酶，也没有影响任何行为变量。然而，B8损伤降低了海马区、隔区、皮质和下丘脑色氨酸羟基酶，其行为影响与中缝联合损伤或对氯苯丙氨酸损伤后的报道相似。中度中缝大鼠在一个新的环境中和整个光明/黑暗周期的黑暗阶段都是过度活跃的。在运动活动方面，B8损伤的大鼠对苯丙胺也有高反应性。当放置在稳定器中并受到反复喷气刺激时，B8损伤的大鼠表现出更大的惊吓反应。此外，只有B8损伤的动物在Y型迷宫中进行了两次未经强化的试验后才能坚持下来。对所有39只动物的所有这些组织学、生化和行为变量进行了单独评估，并结合本论文和前一篇论文的数据进行了多变量相关分析。这些实验表明，起源于B8的中脑边缘5-羟色胺能通路具有抑制行为反应性所必需的一些抑制作用。
The behavior of rats with selective lesions of either the dorsal (B7), median (B8), or lateral (B9) raphe nuclei was compared to that of sham-lesioned controls in a variety of experimental situations. The extent of damage to the midbrain raphe nuclei was determined by fluorescence histochemistry, and the tryptophan hydroxylase and tyrosine hydroxylase activities of 6 forebrain regions were measured for each rat. None of the lesions affected tyrosine hydroxylase activity. Lesions of B7, which reduced tryptophan hydroxylase in the striatum, thalamus, cortex, and hypothalamus, had no significant effect on any of the behavioral measures. Lesions of B9, although twice as large, neither reduced forebrain tryptophan hydroxylase significantly nor affected any of the behavioral variables. However, B8 lesions, which reduced hippocampal, septal, cortical, and hypothalamic tryptophan hydroxylase, had behavioral effects similar to those reported after combined raphe lesions or parachlorophenylalanine. Median raphe-lesioned rats were hyperactive when placed in a novel environment and throughout the dark phase of the light/dark cycle. With respect to locomotor activity, B8-lesioned rats were also hyper-responsive to amphetamine. When placed in a stabilimeter and subjected to repeated air puff stimuli, rats with B8 lesions exhibited larger startle responses. Furthermore, only B8-lesioned animals persevered when given 2 unreinforced trials in a Y-maze. All these histologic, biochemical and behavioral variables were assessed individually for all 39 animals, and a multivariate correlational analysis incorporating the data of this and a preceding paper is presented. These experiments suggest that the mesolimbic serotonergic pathway originating in B8 subserves some of the inhibition necessary to dampen behavioral responsivity.