Effects of the antiandrogens, vinclozolin and cyproterone acetate on gonadal development in the Japanese medaka (Oryzias latipes)
Effects of the antiandrogens, vinclozolin and cyproterone acetate on gonadal development in the Japanese medaka (Oryzias latipes)
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DOI：
10.1016/s0166-445x(02)00189-3
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发表时间：
2003-05-29
影响因子：
4.5
通讯作者：
Metcalf, CD
中科院分区：
文献类型：
--
作者：
Kiparissis, Y;Metcalfe, TL;Metcalf, CD
本研究的重点是确定抗雄激素暴露对日本稻（Oryzias latipes）性腺发育的影响。试验化合物包括杀菌剂vinclozolin和临床抗雄激素醋酸环丙孕酮。新孵化的medaka暴露于vinclozolin（2500马克杯/升）和vinclozolin杀菌剂制剂Ronilan（1000和5000马克杯/升）和cyproterone acetate（1和10马克杯/升）的水溶液中3个月。暴露的鱼的性腺组织的组织学评估表明，5000马克/升浓度的vinclozolin制剂（Ronilan(R)）诱导双性人发生率低。（即睾丸-卵子）和2500杯/升的vinclozolin浓度影响雄性精子的发生。此外，vinclozolin治疗引起中度卵巢闭锁。醋酸环丙孕酮也诱导了低发生率的睾丸-卵子，但与vinclozolin治疗相反，卵巢组织在睾丸-卵子的数量等于或大于睾丸组织的数量。在所有浓度的醋酸环丙孕酮处理下，卵子和精子的发生都受到适度抑制。本研究结果表明，抗雄激素具有改变鱼类睾丸发育和配子体发生的潜力。然而，需要进行研究来确定抗雄激素影响鱼类的机制。(C) 2003 Elsevier Science B.V.版权所有
This study was focused on determining the effects of exposure to antiandrogens on the gonadal development of Japanese medaka (Oryzias latipes). Test compounds included the fungicide, vinclozolin and the clinical antiandrogen, cyproterone acetate. Newly hatched medaka were exposed to aqueous solutions of vinclozolin (2500 mug/l) and the vinclozolin fungicide formulation, Ronilan(R) (1000 and 5000 mug/l) and cyproterone acetate (1 and 10 mug/l), for 3 months. Histological evaluation of the gonadal tissues of exposed fish indicated that the 5000 mug/l concentration of the vinclozolin formulation (Ronilan(R)) induced a low incidence of intersex. (i.e. testis-ova) and the 2500 mug/l concentration of vinclozolin-affected spermatogenesis in males. Also, the vinclozolin treatments induced moderate ovarian atresia. Cyproterone acetate also induced a low incidence of testis-ova, but in contrast to the vinclozolin treatment the amount of ovarian tissue in the testis-ova was equal to or greater than the amount of testicular tissue. In the cyproterone acetate treatments, both oogenesis and spermatogenesis were moderately inhibited at all test concentrations. The results of this study indicate that antiandrogens have the potential to alter testicular development and gametogenesis in fish. However, research is needed to determine the mechanisms by which antiandrogens affect fish. (C) 2003 Elsevier Science B.V. All rights reserved.