Rapid Increase of CTX-M-Producing Shigella sonnei Isolates in Switzerland Due to Spread of Common Plasmids and International Clones
Rapid Increase of CTX-M-Producing Shigella sonnei Isolates in Switzerland Due to Spread of Common Plasmids and International Clones
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DOI：
10.1128/aac.01057-20
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发表时间：
2020-10-01
影响因子：
4.9
通讯作者：
Endimiani, Andrea
中科院分区：
文献类型：
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作者：
Campos-Madueno, Edgar, I;Bernasconi, Odette J.;Endimiani, Andrea
瑞士抗生素耐药性中心 (ANRESIS) 最近注意到全国范围内的超广谱头孢菌素耐药 (ESC-R) 宋内志贺菌分离株有所增加（2016 年为 3.8%，2019 年为 37.5%）。为了理解这一现象，我们分析了 2016 年至 2019 年在瑞士收集的 25 个代表性分离株（其中 14 个为 ESC-R）。使用 Illumina 和 Nanopore 平台实现了全基因组测序。 ESC-R 和超广谱头孢菌素敏感分离株均属于序列类型 152 (ST152)。 ESC-R 分离株在 IncI1-pST57 (n = 5) 中携带 bla(CTX-M-3)，在 IncFII (F2:A-:B-) (n = 5) 中携带 bla(CTX-M-15)，在 IncI1-pST16 中携带 bla(CTX-M-15)，以及 bla(CTX-M-27)、bla(CTX-M-55) 或其他 IncFII 质粒中的 bla(CTX-M-134)（每个 n = 1）。具有相同bla和Inc组的质粒彼此之间表现出高度的遗传同一性，而且与先前在其他肠杆菌目中报道的质粒也表现出高度的遗传同一性。核心基因组分析显示，有 4 个主要簇，每个簇都包含不同程度的菌株
The Swiss Centre for Antibiotic Resistance (ANRESIS) has recently noted an increase of extended-spectrum cephalosporin-resistant (ESC-R) Shigella sonnei isolates nationwide (3.8% in 2016 versus 37.5% in 2019). To understand this phenomenon, we analyzed 25 representative isolates (of which 14 were ESC-R) collected in Switzerland during 2016 to 2019. Whole-genome sequencing was achieved using both the Illumina and the Nanopore platforms. Both ESC-R and extended-spectrum cephalosporin-susceptible isolates belonged to sequence type 152 (ST152). The ESC-R isolates carried bla(CTX-M-3) in IncI1-pST57 (n = 5), bla(CTX-M-15) in IncFII (F2:A-:B-) (n = 5), bla(CTX-M-15) in IncI1-pST16, and bla(CTX-M-27), bla(CTX-M-55), or bla(CTX-M-134) in other IncFII plasmids (n = 1 each). Plasmids having the same bla and Inc group exhibited high degrees of genetic identity to each other but also to plasmids previously reported in other Enterobacterales. Core-genome analysis showed that there were 4 main clusters, each of which included strains that differed by