Assessment of the rind microbial diversity in a farm house-produced vs a pasteurized industrially produced soft red-smear cheese using both cultivation and rDNA-based methods
Assessment of the rind microbial diversity in a farm house-produced vs a pasteurized industrially produced soft red-smear cheese using both cultivation and rDNA-based methods
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DOI：
10.1111/j.1365-2672.2004.02333.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Vallaeys, T
中科院分区：
文献类型：
--
作者：
Feurer, C;Irlinger, F;Vallaeys, T
目的：两个法国红涂片软奶酪的表面植物群的多样性进行了检查，培养依赖和培养独立的方法，以评估其组成，并评估这两种approachs.Methods和Results的准确性：培养独立的方法涉及16 S核糖体DNA基因克隆和测序和单链构象多态性分析（SSCP）。所用的培养依赖性方法包括直接培养和肉眼观察，对从单菌落中提取的DNA中的16S rRNA基因进行聚合酶链反应，然后对基因进行完整测序。无论是巴氏灭菌奶酪还是农家奶酪，这两种方法都只能回收到很少的物种。在两种奶酪的表面鉴定出了大量的海洋细菌相关的分离物或16S rDNA序列。结论：结果表明，所有三种技术都提供了信息和互补性，可以更准确地代表奶酪表面的生物多样性。培养和分子方法必须结合起来，以获得复杂生态系统的细菌种群的扩展视图。
Aims: The diversity of the surface flora of two French red-smear soft cheeses was examined by cultivation-dependent and cultivation-independent methods to assess their composition and to evaluate the accuracy of both approaches.Methods and Results: Culture-independent methods used involved 16S ribosomal DNA gene cloning and sequencing and single-strand conformation polymorphism analysis (SSCP). The culture-dependent method used involved direct culture and macroscopic observation, polymerase chain reaction of the 16S rRNA gene from DNA extracted from single colonies followed by complete sequencing of the gene. Only few species were recovered by both approaches either in the pasteurized and the farmer cheese. A large diversity of isolates or 16S rDNA sequences related to marine bacteria was identified at the surface of both cheeses.Conclusions: The results indicated that all three techniques were informative and complementary to allow a more accurate representativeness of the cheese surface biodiversity.Significance and Impact of the Study: Cultivation and molecular methods have to be combined in order to obtain an extended view of the bacterial populations of complex ecosystems.