ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells
ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells
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DOI：
10.1242/jcs.062034
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发表时间：
2010-04-01
影响因子：
4
通讯作者：
Chow, Kuan-Chih
中科院分区：
文献类型：
--
作者：
Fang, Hsin-Yuan;Chang, Chia-Ling;Chow, Kuan-Chih
含AAA结构域的3A(ATAD3A)是AAA-ATPase家族的成员。ATAD3有三种形式：ATAD3A、ATAD3B和ATAD3C。在本研究中，我们检测了ATAD3在肺腺癌中的类型和表达。用逆转录聚合酶链式反应、免疫印迹、免疫组织化学和共聚焦免疫荧光显微镜检测ATAD3A的表达。我们的结果表明ATAD3A是LADC表达的主要形式。抑制ATAD3A的表达增加了线粒体碎裂和顺铂的敏感性。血清剥夺增加了ATAD3A的表达和耐药性。这些结果提示，ATAD3A可能是LADC的抗凋亡标志物。
AAA domain-containing 3A (ATAD3A) is a member of the AAA-ATPase family. Three forms of ATAD3 have been identified: ATAD3A, ATAD3B and ATAD3C. In this study, we examined the type and expression of ATAD3 in lung adenocarcinoma (LADC). Expression of ATAD3A was detected by reverse transcription-polymerase chain reaction, immunoblotting, immunohistochemistry and confocal immunofluorescent microscopy. Our results show that ATAD3A is the major form expressed in LADC. Silencing of ATAD3A expression increased mitochondrial fragmentation and cisplatin sensitivity. Serum deprivation increased ATAD3A expression and drug resistance. These results suggest that ATAD3A could be an anti-apoptotic marker in LADC.