In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation
In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation
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DOI：
10.1089/clo.2006.8.159
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发表时间：
2006-09-01
期刊：
影响因子：
--
通讯作者：
Yoshida, Mitsutoshi
中科院分区：
文献类型：
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作者：
Miyoshi, Kazuchika;Sato, Keisuke;Yoshida, Mitsutoshi
本研究研究了将小型猪体细胞移植到农场猪去核卵母细胞中，经超声照射后克隆胚胎的激活和发育情况。超声作用下胚胎原核样结构的形成和极体样结构的挤压率与电脉冲作用下胚胎的形成率无显著差异。虽然不同激活方式的囊胚形成率无显著差异，但超声激活的囊胚平均细胞数显著高于电脉冲激活的囊胚数量（p < 0.05）。本研究结果表明，超声刺激可诱导小型猪体细胞克隆胚胎的激活和体外发育。
The present study was carried out to examine the activation and development of cloned embryos produced by transferring miniature pig somatic cells into enucleated farm pig oocytes after exposing to ultrasound. The rates of the pronucleus-like structure formation and polar body-like structure extrusion in embryos exposed to ultrasound did not differ from those applied electric pulses. Although there was no significant difference in the blastocyst formation rates between different activation methods, the mean number of cells in the blastocysts developed from embryos activated by exposing to ultrasound was significantly (p < 0.05) higher than that obtained by applying electric pulses. The results of the present study showed that ultrasound stimulation can induce the activation and in vitro development of cloned embryos derived from miniature pig somatic cells.