Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312
Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312
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DOI：
10.1016/j.carres.2014.06.011
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发表时间：
2015-02-11
影响因子：
3.1
通讯作者：
Holst, Otto
中科院分区：
文献类型：
--
作者：
Turska-Szewczuk, Anna;Pietras, Hubert;Holst, Otto
采用糖基化和甲基化分析沿着H-1和C-13核磁共振谱，包括2D H-1、H-1 COSY、TOCSY、NOESY、H-1检测的H-1、C-13 HSQC和HMBC实验，对温和气单胞菌Pt 312菌株脂多糖进行了研究。使用H-1、H-1 NOESY和H-1、C-13 HMBC实验确定糖残基的序列。发现OPS由GlcpNAc和非化学计量的0-乙酰化的Rhap残基组成的二糖重复单元构成，并且具有以下结构：4）-α-L-Rhap-（1 -> 3）-β-D-GlcpNAc-（1 -> 2/3|类似于80/35%0Ac（C）2014爱思唯尔有限公司。保留所有权利。
The O-specific polysaccharide (OPS) obtained by mild-acid degradation of the lipopolysaccharide from Aeromonas sobria strain Pt312 was studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including 2D H-1, H-1 COSY, TOCSY, NOESY, H-1-detected H-1, C-13 HSQC, and HMBC experiments. The sequence of the sugar residues was determined using H-1, H-1 NOESY and H-1, C-13 HMBC experiments. It was found that the OPS was built up of disaccharide repeating units composed of GlcpNAc and non-stoichiometrically O-acetylated Rhap residues, and had the structure.-> 4)-alpha-L-Rhap-(1 -> 3)-beta-D-GlcpNAc-(1 -> 2/3 |similar to 80/35% OAc (C) 2014 Elsevier Ltd. All rights reserved.