ANTIOXIDANT SYSTEMS IN SCHISTOSOMA-MANSONI - CORRELATION BETWEEN SUSCEPTIBILITY TO OXIDANT KILLING AND THE LEVELS OF SCAVENGERS OF HYDROGEN-PEROXIDE AND OXYGEN FREE-RADICALS
ANTIOXIDANT SYSTEMS IN SCHISTOSOMA-MANSONI - CORRELATION BETWEEN SUSCEPTIBILITY TO OXIDANT KILLING AND THE LEVELS OF SCAVENGERS OF HYDROGEN-PEROXIDE AND OXYGEN FREE-RADICALS
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DOI：
10.1016/0020-7519(88)90101-4
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发表时间：
1988-07-01
影响因子：
4
通讯作者：
PRICHARD, RK
中科院分区：
文献类型：
--
作者：
MKOJI, GM;SMITH, JM;PRICHARD, RK
The relative susceptibility of Schistosoma mansoni schistosomula and adults to cell-free oxidant mediated killing has been studied and correlated with levels of oxidant scavengers in the parasite stages. Over 95% of the schistosomula were killed in H2O2 (0.25 mM) or H2O2 generated by glucose (5.5 mM)-glucose oxidase (5 mU ml-1); and in superoxide, H2O2, and possibly hydroxyl radical and singlet oxygen, generated by xanthine (0.5 mM)- or acetaldehyde (0.5 mM)-xanthine oxidase (10 mU ml-1). Under similar conditions less than 2% of the adults were killed. The oxidant scavenger enzymes superoxide dismutase, cytochrome c peroxidase, glutathione peroxidase, glutathione reductase, and the tripeptide glutathione were present in the parasite, but not the enzyme catalase. Enzyme activities and glutathione levels were significantly lower in the schistosomula than adult worms. Juvenile and adult S. mansoni show differences in susceptibility to in vitro oxidant killing that correlate with differences in their levels of endogenous antioxidants.
