Cell-cell communication between adipocytes and pancreatic beta-cells in acoustically levitated droplets.
Cell-cell communication between adipocytes and pancreatic beta-cells in acoustically levitated droplets.
复制标题
声悬浮液滴中脂肪细胞和胰腺β细胞之间的细胞间通讯。
作者：
S. Santesson;E. Degerman;P. Rorsman;T. Johansson;Sandra Lemos;S. Nilsson
功能失调的脂肪细胞和产生胰岛素的胰岛β细胞是人类2型糖尿病的特征，在疾病的发生和发展中发挥着重要作用。然而，所涉及的确切机制是复杂的，而且只有部分人了解。在这里，我们提出了一种新的、独特的方法，在2型糖尿病相关研究中进行单细胞和细胞间通讯研究。机载分析系统提供了亚微升体积范围内的非接触式样品处理，并配备了荧光成像检测。该系统使用声学悬浮液滴作为“无壁”试管，内部构造的压电式流通式皮升液滴分配器用于精确的试剂供应。激素对脂肪细胞脂解的调节以及脂肪细胞和β细胞之间的通讯可以在少数细胞水平上进行研究。因此，无论是异丙肾上腺素诱导的还是胰岛素抑制的，在单个脂肪细胞中都可以检测到脂解。此外，空气传播系统可以比较不同大小的脂肪细胞的脂肪分解情况：大脂肪细胞的反应比小细胞慢。此外，高糖或乙酰胆碱对含有胰岛素产生的β细胞的悬浮滴状细胞的胰岛素分泌的刺激，导致抑制异丙肾上腺素诱导的脂肪细胞在同一滴状中的脂肪分解。结果表明，气载分析系统适用于单细胞分析和细胞间通讯研究，以及未来直接从临床活检获得的人类细胞进行分析的可能性。
Dysfunctional adipocytes and insulin-producing pancreatic beta-cells are hallmarks of human Type 2 diabetes and play important roles in the onset and progression of the disease. However, the precise mechanisms involved are complex and only partially understood. Here we present a new and unique method to perform single-cell and cell-cell communication studies in Type 2 diabetes-related research. The airborne analytical system offers "contactless" sample handling in the sub-microlitre volume range and is here equipped with fluorescence imaging detection. The system utilizes acoustically levitated droplets as "wall-less" test tubes and in-house constructed piezoelectric flow-through picolitre droplet dispensers for precise reagent supply. Hormone-mediated regulation of adipocyte lipolysis and communication between adipocytes and beta-cells can be studied at the few-cell level. Thus, lipolysis could be detected in single adipocytes, whether it was induced by isoprenaline or inhibited by insulin. Furthermore, the airborne system allowed the comparison of lipolysis in adipocytes of different sizes: a large adipocyte responded more slowly than a small cell. Furthermore, stimulation of insulin secretion by high glucose or acetylcholine administration to a levitated drop containing insulin-producing beta-cells resulted in inhibition of isoprenaline-induced lipolysis in adipocytes present in the same drop. The results show the applicability of the airborne analytical system for single cell analysis and for cell-cell communication studies as well as the potential for future analysis directly from human cells obtained from clinical biopsies.
影响因子：
7.4
作者：
Ni, Qihui;Reid, Kendra R.;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.