Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding.
Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding.
复制标题
Dickerson-Drew 十二聚体中阳离子链的结合：能量学、结构以及离子和水结合的相关性。
DOI：
10.1021/bi050897i
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Marky,LuisA
中科院分区：
文献类型：
--
作者：
Shikiya,Ronald;Li,Jian-Sen;Gold,Barry;Marky,LuisA
We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson−Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2‘-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contributions due to the removal of electrostricted water and higher exposure of polar and nonpolar atomic groups that immobilize structural water. These cationic chains effectively remove counterions from the major groove, neutralizing some negatively charged phosphates. The overall results are consistent with the NMR solution of the modified duplex that showed a small bend at each modified site.
