Tri-layered elastomeric scaffolds for engineering heart valve leaflets.
Tri-layered elastomeric scaffolds for engineering heart valve leaflets.
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DOI：
10.1016/j.biomaterials.2014.04.039
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发表时间：
2014-09
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Masoumi, Nafiseh;Annabi, Nasim;Assmann, Alexander;Larson, Benjamin L;Hjortnaes, Jesper;Alemdar, Neslihan;Kharaziha, Mahshid;Manning, Keefe B;Mayer, John E Jr;Khademhosseini, Ali
可以生长和重塑的组织工程心脏瓣膜（TEHV）有可能作为目前无活力人工瓣膜的永久替代品，特别是对于儿科患者。设计功能性TEHV的主要挑战是模拟天然瓣叶的结构和各向异性机械特性。为了建立一个更加仿生的TEHV模型，我们采用静电纺丝和微细加工技术相结合的方法制备了三层结构的支架。这些结构是通过组装微加工的聚（癸二酸甘油酯）（PGS）和纤维PGS/聚（-己内酯）（PCL）电纺片开发弹性支架与可调各向异性的机械性能类似的天然心脏瓣膜的机械特性。工程支架支持瓣膜间质细胞（VIC）和间充质干细胞（MSC）在3D结构内生长，并促进心脏瓣膜细胞外基质（ECM）的沉积。MSC也沿着工程化的三层支架的各向异性轴组织和排列沿着。此外，制造的结构在猪心脏瓣膜瓣叶组织置换的离体模型中正确打开和闭合。工程化的三层支架具有成功转化为TEHV替代物的潜力。
Tissue engineered heart valves (TEHVs) that can grow and remodel have the potential to serve as permanent replacements of the current non-viable prosthetic valves particularly for pediatric patients. A major challenge in designing functional TEHVs is to mimic both structural and anisotropic mechanical characteristics of the native valve leaflets. To establish a more biomimetic model of TEHV, we fabricated tri-layered scaffolds by combining electrospinning and microfabrication techniques. These constructs were fabricated by assembling microfabricated poly(glycerol sebacate) (PGS) and fibrous PGS/poly(-caprolactone) (PCL) electrospun sheets to develop elastic scaffolds with tunable anisotropic mechanical properties similar to the mechanical characteristics of the native heart valves. The engineered scaffolds supported valvular interstitial cells (VICs) and mesenchymal stem cells (MSCs) growth within the 3D structure and promoted the deposition of heart valve extracellular matrix (ECM). MSCs were also organized and aligned along the anisotropic axes of the engineered tri-layered scaffolds. In addition, the fabricated constructs opened and closed properly in an ex vivo model of porcine heart valve leaflet tissue replacement. The engineered tri-layered scaffolds have the potential for successful translation towards TEHV replacements.