Xenograft models for liver metastasis: Relationship between tumor morphology and adenovirus vector transduction
Xenograft models for liver metastasis: Relationship between tumor morphology and adenovirus vector transduction
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DOI：
10.1016/j.ymthe.2004.01.021
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发表时间：
2004-05-01
影响因子：
12.4
通讯作者：
Lieber, A
中科院分区：
文献类型：
--
作者：
Li, ZY;Ni, SH;Lieber, A
利用病毒载体提高肿瘤细胞的初始转导能力是肿瘤基因治疗的主要任务。我们建立了肝转移的小鼠肿瘤模型，以研究全身腺病毒载体应用后肿瘤细胞的转导。将人肿瘤细胞系移植到免疫缺陷小鼠体内，建立肿瘤模型。在尾静脉注射腺病毒载体后，分析了来自宫颈、结肠癌、乳腺癌和肝癌的肝转移瘤细胞外基质的分布、血管形成和转基因表达。总体而言，异种移植与癌症患者相应肿瘤的形态相似。腺病毒介导的基因传递依赖于肿瘤血管的形成和血管与肿瘤细胞的直接接触。这些模型是研究和改进肿瘤基因治疗方法的重要工具。
The improvement of initial tumor cell transduction with viral vectors is a major task in tumor gene therapy. We have developed mouse tumor models with hepatic metastases to study transduction of tumor cells after systemic adenovirus vector application. The tumor models were established by intraportal transplantation of human tumor cell lines into immunodeficient mice. Liver metastases derived from cervix, colon, breast, and liver cancer lines were analyzed for distribution of extracellular matrix, vascularization, and transgene expression after tail vein injection of adenovirus vectors. Overall, xenografts resembled the morphology of corresponding tumors in cancer patients. Adenovirus-mediated gene delivery depended on tumor vascularization and direct contact between blood vessels and tumor cells. These models represent important tools for studying and improving tumor gene therapy approaches.