The differential expression of a heat shock promoter in floral and reproductive tissues
The differential expression of a heat shock promoter in floral and reproductive tissues
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DOI：
10.1046/j.1365-3040.2001.00727.x
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发表时间：
2001-08-01
影响因子：
7.3
通讯作者：
Mascarenhas, JP
中科院分区：
文献类型：
--
作者：
Crone, D;Rueda, J;Mascarenhas, JP
大豆小热激蛋白基因启动子，GmHSP 17.5E，融合到GUS报告基因的表达的详细组织化学分析，已在所有器官和组织的花作为一个功能的发展阶段和热胁迫。该启动子在热激后在所有花组织和器官中不均匀地表达。在萼片的所有发育阶段都可以看到表达，但在花瓣中看不到。在雌蕊和花药中的表达模式是复杂的。热胁迫诱导的GUS染色见于花柱和子房上部，但不见于柱头乳突、子房下部或胚珠。在雄蕊中，在花丝和维管组织从花丝向花药的延伸中可以看到热激反应。在花药的其他组织或在任何发育阶段的小孢子或花粉中没有观察到诱导。而营养器官在GUS活性的电击诱导中表现得更为均匀。基于来自微粒轰击GmHSP 17.5E/GUS构建体进入花粉后的瞬时测定的证据，在稳定转化的转基因植物中，该基因在花粉核中可能在转录上处于失活构型。这些结果进行了讨论，参考文献中的其他信息。
A detailed histochemical analysis of the expression of the soybean small heat shock protein gene promoter, GmHSP 17.5E, fused to the GUS reporter gene, has been made in all organs and tissues of the flower as a function of stage of development and heat stress. This promoter is not uniformly expressed after a heat shock in all floral tissues and organs. Expression is seen at all stages of development in the sepals but not in the petals. The expression pattern in the pistil and in anthers is complex. Heat stress-induced GUS staining is seen in the style and upper portion of the ovary, but not in the stigmatic papillae or in the lower part of the ovary or in ovules. In stamens the heat shock response is seen in the filament and in the extension of the vascular tissue from the filament into the anther. No induction is seen in other tissues of the anther or in microspores or pollen at any stage of development. Vegetative organs in contrast are more uniform in the beat shock inducibility of GUS activity. Based on evidence from transient assays after microprojectile particle bombardment of the GmHSP 17.5E/GUS construct into pollen, it is likely that the gene is transcriptionally in an inactive configuration in pollen nuclei in stably transformed transgenic plants. These results are discussed with reference to other information in the literature.