Nucleotide sequence of the gene for the gamma chain of human fibrinogen.
Nucleotide sequence of the gene for the gamma chain of human fibrinogen.
复制标题
人纤维蛋白原γ链基因的核苷酸序列。
DOI：
10.1021/bi00329a041
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Davie,EW
中科院分区：
文献类型：
--
作者：
Rixon,MW;Chung,DW;Davie,EW
Mark W. Rixon,* Dominic W. Chung, and Earl W. Davie* Department of Biochemistry, University of Washington, Seattle, Washington 98195 Received October 5, 1984 abstract: A human genomic DNA library was screened for the gene coding for the y chain of fibrinogen by using a human cDNA for the y chain as a hybridization probe. The gene was identified in three overlapping recombinant\bacteriophage, and its sequence, including the immediate 5'and 3'flanking regions, was determined. The DNA sequence analysis revealed the presence of 10 exons coding for 411 amino acids present in the mature protein and a signal sequence of 26 amino acids. Two 30 base pair (bp) direct repeats of 93% identity were found 468 bp upstream from the transcription initiation site. The DNA sequence of the gene for the y chain of human fibrinogen showed considerable sequence homology with a partial sequence reported for the gene for the y chain of rat fibrinogen.Fibrinogen is a plasma glycoproteinthat participates in the final stage of blood coagulation [for a recent review see Doolittle (1984)]. Fibrinogen (Mr 340000) consists of pairs of three different polypeptide chains, designated a, f3, and y (McKee et al., 1966). 1 Thrombin releases fibrinopeptides A and B from the amino termini of the a and/3 chains, re-spectively, converting fibrinogen to fibrin monomers. The fibrin monomers then polymerize and are covalently cross-linked by factor XHIa to form a tough insoluble fibrin clot (Doolittle, 1973, 1975).
