PROTEIN ADSORPTION STUDIES ON STANDARD POLYMERIC MATERIALS
PROTEIN ADSORPTION STUDIES ON STANDARD POLYMERIC MATERIALS
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DOI：
10.1163/156856293x00816
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发表时间：
1993-01-01
影响因子：
3.6
通讯作者：
TENHOVE, P
中科院分区：
文献类型：
--
作者：
BRASH, JL;TENHOVE, P
研究了纤维蛋白原作为单一蛋白从缓冲液和血浆中吸附到四种材料上的情况。两个NIH-NHLBI主要参考标准，无填料聚二甲基硅氧烷和低密度聚乙烯，与IUPAC工作组提供的聚氯乙烯和纤维素材料一起使用。除聚二甲基硅氧烷仅以管状形式进行研究外，这些材料以薄膜和管状形式进行了研究。室温下用i -125标记纤维蛋白原测定吸附量。单蛋白吸附量的大小顺序为：纤维素< PVC < PE = PDMS。表观吸附亲和度按相同顺序排列。在血浆中，除纤维素外的所有表面在接触5min后均表现出最大的吸附量。这表明最初的吸附随后是纤维蛋白原的位移（Vroman效应）。纤维素对血浆中纤维蛋白原的吸附率很低。Vroman最大值在管材样品上比在薄膜样品上更明显，并且，对于单蛋白实验，发现管材样品的吸附比薄膜样品少。对Vroman效应数据的初步解释表明，材料的促凝剂活性的顺序可能是：PDMS = PE < PVC <纤维素。
Adsorption of fibrinogen from buffer as a single protein and from plasma to four materials has been studied. The two NIH-NHLBI primary reference standards, filler free polydimethylsiloxane and low density polyethylene, were used along with polyvinylchloride and cellulose materials supplied by the IUPAC Working Party. The materials were examined in both film and tubing form, except for polydimethylsiloxane which was studied only in tubing form. Adsorption was measured at room temperature using I-125-labelled fibrinogen. The order of adsorbed amounts in the single protein experiments was found to be: cellulose < PVC < PE = PDMS. Apparent adsorption affinities are in the same order. In plasma, all surfaces except cellulose showed maxima in adsorption as a function of plasma concentration after 5 min contact. This is indicative of initial adsorption followed by displacement of fibrinogen (the Vroman effect). Cellulose showed very low adsorption of fibrinogen from plasma. The Vroman maxima were more pronounced on the tubing samples than on the films, and, as for the single protein experiments, adsorption was found to be less on tubing than on film samples. A tentative interpretation of the Vroman effect data suggests that the order of procoagulant activity of the materials may be: PDMS = PE < PVC < cellulose.