Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2
Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2
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DOI：
10.1007/bf00021807
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发表时间：
1996-05-01
影响因子：
5.1
通讯作者：
Moroney, JV
中科院分区：
文献类型：
--
作者：
Burow, MD;Chen, ZY;Moroney, JV
单细胞藻类在有限的CO2条件下生长良好，这得益于碳浓缩机制（CCM）。In C. reinhardtii，这种机制是可诱导的，只存在于低CO2条件下生长的细胞中。我们构建了一个低CO2适应细胞的cDNA文库，并筛选了低CO2适应细胞特异性cDNA文库。6类低CO2诱导的克隆进行了鉴定。本文报道的一类克隆代表了一种与细胞适应空气有关的新基因。第二类克隆对应于空气诱导的周质碳酸酐酶I（CAH1）。这些克隆代表了对环境中二氧化碳水平做出反应的基因。
Unicellular algae grow well under limiting CO, conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism as inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.