Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen
Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen
复制标题
DOI：
10.1073/pnas.041625098
复制
发表时间：
2001-03-13
影响因子：
11.1
通讯作者：
Nakayama, E
中科院分区：
文献类型：
--
作者：
Ono, T;Kurashige, T;Nakayama, E
同源小鼠肉瘤引起体液免疫应答的抗原的血清学表达克隆鉴定出pem（小鼠胎盘和胚胎表达基因）是癌症/睾丸家族的新成员。为了鉴定pem在人体内的同源性，我们利用人睾丸中小鼠pem序列和pem相关表达序列标签作为PCR引物，利用人睾丸cDNA进行扩增。然而，与pem不同的是，另一个被命名为y - es -1的基因被分离出来，并被发现是最初在小鼠、豚鼠和猪中发现的原克rossin结合蛋白sp32前体的人类同源物。y - tes -1定位于12p12-p13染色体，包含10个外显子。Southern blot分析表明，在人类基因组中存在两个oy - tes -1相关基因。在正常组织中，y - tes -1 mRNA仅在睾丸中表达，而在恶性组织中，多种癌症，包括膀胱癌、乳腺癌、肺癌、肝癌和结肠癌，都表达y - tes -1。对362例不同类型肿瘤患者进行血清学调查，其中25例检测出OY-TES-1抗体。20例正常人血清无y - tes -1反应。这些发现表明，y - tes -1是癌症/睾丸抗原家族的一个额外成员，并且y - tes -1在人类中具有免疫原性。
Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new mem ber of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans.