Culture-based and sequence-based insights into biosynthesis of secondary metabolites by Aspergillus terreus ATCC 20542
Culture-based and sequence-based insights into biosynthesis of secondary metabolites by Aspergillus terreus ATCC 20542
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DOI：
10.1016/j.jbiotec.2014.01.038
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发表时间：
2014-04-10
影响因子：
4.1
通讯作者：
Bizukojc, Marcin
中科院分区：
文献类型：
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作者：
Boruta, Tomasz;Bizukojc, Marcin
为了激活其基因组编码的生物合成途径和探索次生代谢谱，在不同的培养基中培养了土曲霉ATCC 20542。除甲氧乙烯酸(洛伐他汀)及其前体莫纳可林L外，在所分析的培养物中还发现了许多其他次生代谢物，即土酸、桔霉素、(+)-地黄素、土豆素和脱氢苦参素。与已报道的与洛伐他汀、莫纳可林L、(+)-地黄素和脱氢苦参素的产生相关的基因簇不同，土曲霉与土酸、桔霉素和地黄素的形成相关的基因簇仍有待鉴定。在可公开获得的Terreus NIH2624基因组中，推测可能与桔霉素和土酸的生物合成有关的基因簇。通过将注释结果与已提出的生物合成途径和已发表的关于潜在酶的生化研究相对照，预测了先前已确定的(+)-geodin生物合成簇中可能的基因的功能。由于目前还没有关于土壤素生物合成的遗传方面的数据，因此没有提出可能对土壤素的生产负责的候选基因簇。(C)2014爱思唯尔B.V.保留所有权利。
Aspergillus terreus ATCC 20542 was cultivated in various culture media in order to activate its genome-encoded biosynthetic pathways and explore the secondary metabolic repertoire. In addition to mevinolinic acid (lovastatin) and its precursor monacolin L, a number of other secondary metabolites were found in the analyzed cultures, namely terreic acid, citrinin, (+)-geodin, terrein, and dehydrocurvularin. In contrast to previously described gene clusters responsible for production of lovastatin, monacolin L, (+)-geodin and dehydrocurvularin, the gene clusters of A. terreus associated with the formation of terreic acid, citrinin and terrein still await identification. Putative gene clusters potentially related to citrinin and terreic acid biosynthesis were suggested in the publicly available genome of A. terreus NIH 2624. The functions of putative genes in the previously identified cluster of (+)-geodin biosynthesis were predicted by confronting the annotation results with the proposed biosynthetic pathway and published biochemical studies on the underlying enzymes. Since there were no available data regarding genetic aspects of terrein biosynthesis, the candidate gene cluster potentially responsible for the production of terrein was not suggested. (C) 2014 Elsevier B.V. All rights reserved.