MHC and kin discrimination in juvenile Arctic charr, Salvelinus alpinus (L.)
MHC and kin discrimination in juvenile Arctic charr, Salvelinus alpinus (L.)
复制标题
DOI：
10.1006/anbe.1998.0837
复制
发表时间：
1998-08-01
期刊：
影响因子：
2.5
通讯作者：
Langefors, Å
中科院分区：
文献类型：
--
作者：
Olsén, KH;Grahn, M;Langefors, Å
亲属识别和辨别被认为发生在不同分类群的几个物种中。在鲑科鱼类中，幼鱼可以区分来自同一种群的兄弟姐妹和非兄弟姐妹的气味，即使气味供体和测试鱼自受精以来是分开饲养的。这表明某些遗传因素在识别过程中起着重要作用。亲属识别和歧视背后的机制尚未被描述。在本研究中，我们进行了河道试验，以检验幼鱼北极鲑的亲缘识别和辨别是否受到主要组织相容性复合体（MHC）的影响。在fluviarium试验之前，用变性梯度凝胶电泳（DGGE）分析了charr中MHC II类B基因的外显子2，并确定了个体基因型。在河流养殖场中，当鱼在MHC相同的兄弟姐妹和MHC基因型不同的兄弟姐妹之间做出选择时，它们更喜欢来自相同兄弟姐妹的水。此外，来自MHC不同兄弟姐妹的水比来自MHC不同的非兄弟姐妹的水更受欢迎。然而，当测试鱼与非兄弟姐妹供体共享一个等位基因而与兄弟姐妹供体没有等位基因时，我们没有观察到歧视。我们的研究结果表明，MHC对北极炭幼鱼用于亲属识别和区分的气味有显著影响。(C) 1998动物行为研究协会。
Kin recognition and discrimination ale thought to occur in several species of various taxonomic groups. In salmonid fish, juveniles can discriminate between odours of siblings and nonsiblings from the same population even if the odour donors and the test fish have been reared separately since fertilization. This indicates that some genetic factor is important in the recognition process. The mechanisms behind kin recognition and discrimination have not yet been described. In the present study, we performed fluviarium tests to examine whether kin recognition and discrimination in juvenile Arctic charr are influenced by the major histocompatibility complex (MHC). Prior to the fluviarium tests, exon 2 of an MHC class II B gene in charr was analysed with denaturing gradient gel electrophoresis (DGGE) and individual genotypes were determined. In the fluviarium, when fish had the choice between water scented by an MHC identical sibling: and a sibling with a different MHC genotype they preferred water from identical siblings. Moreover, water scented by an MHC different sibling was preferred to water from an MHC different nonsibling. However, we observed no discrimination when the test fish shared one allele with the nonsibling donor but no alleles with the sibling donor. Our results indicate that the MHC has a significant influence on the odours used for kin recognition and discrimination in juvenile Arctic charr. (C) 1998 The Association for the Study of Animal Behaviour.