A Simple Analysis of Purine and Pyrimidine Nucleotides in Plant Cells by High-Performance Liquid Chromatography
A Simple Analysis of Purine and Pyrimidine Nucleotides in Plant Cells by High-Performance Liquid Chromatography
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高效液相色谱法简单分析植物细胞中的嘌呤和嘧啶核苷酸
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
T. Ukaji
中科院分区：
文献类型：
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作者：
H. Ashihara;Kaori Mitsui;T. Ukaji
摘要用6%高氯酸从培养的植物细胞中提取嘌呤和嘧啶核苷酸，用阴离子交换树脂Shimpack WAX-1柱进行高效液相色谱分离。超过15个核苷单，二，三磷酸和核苷酸糖清楚地分离和定量，没有任何干扰植物酚类化合物。
Abstract Purine and pyrimidine nucleotides, extracted from cultured plant cells with 6 % perchloric acid, were separated directly with HPLC using anion-exchange Shimpack WAX -1 column. More than fifteen nucleoside mono-, di-, and triphosphates and nucleotide sugars were clearly separated and quantified without any interference from plant phenolic compounds.