Diversity of Arbuscular Mycorrhizal Fungus Populations in Heavy-Metal-Contaminated Soils
Diversity of Arbuscular Mycorrhizal Fungus Populations in Heavy-Metal-Contaminated Soils
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DOI：
10.1128/aem.65.2.718-723.1999
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发表时间：
1999-02
影响因子：
4.4
通讯作者：
C. Val;J. Barea;C. Azcón-Aguilar
中科院分区：
文献类型：
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作者：
C. Val;J. Barea;C. Azcón-Aguilar
摘要高浓度的重金属污染对土壤中微生物种群的数量、多样性和活性有不利影响。本工作的目的是确定如何的丛枝菌根（AM）真菌的多样性的影响，除了污水中含有重金属的修正污泥在长期的实验。由于实验土壤中土著AM真菌（AMF）繁殖体的数量减少，因此使用了几种具有不同生活史的宿主植物来繁殖土著真菌。六AMF生态型被发现在实验土壤中，表现出一致的差异，就其耐受重金属的存在。AMF生态类型范围从非常敏感的金属的存在相对耐受土壤中的重金属含量高。随着土壤中重金属含量的增加，AMF孢子总数呈下降趋势。然而，物种丰富度和多样性的Shannon-Wiener指数增加，在土壤中接受中等速率的污泥污染，但减少在土壤中接受最高速率的重金属污染的污泥。大多数AMF物种的相对密度也显着影响土壤处理。寄主植物种类对AMF种群大小和多样性有选择性影响。我们得出结论，根据这项研究的结果，AMF种群的大小和多样性被修改的金属污染的土壤中，即使在那些金属浓度低于欧盟接受的农业土壤的上限。
ABSTRACT High concentrations of heavy metals have been shown to adversely affect the size, diversity, and activity of microbial populations in soil. The aim of this work was to determine how the diversity of arbuscular mycorrhizal (AM) fungi is affected by the addition of sewage-amended sludge containing heavy metals in a long-term experiment. Due to the reduced number of indigenous AM fungal (AMF) propagules in the experimental soils, several host plants with different life cycles were used to multiply indigenous fungi. Six AMF ecotypes were found in the experimental soils, showing consistent differences with regard to their tolerance to the presence of heavy metals. AMF ecotypes ranged from very sensitive to the presence of metals to relatively tolerant to high rates of heavy metals in soil. Total AMF spore numbers decreased with increasing amounts of heavy metals in the soil. However, species richness and diversity as measured by the Shannon-Wiener index increased in soils receiving intermediate rates of sludge contamination but decreased in soils receiving the highest rate of heavy-metal-contaminated sludge. Relative densities of most AMF species were also significantly influenced by soil treatments. Host plant species exerted a selective influence on AMF population size and diversity. We conclude based on the results of this study that size and diversity of AMF populations were modified in metal-polluted soils, even in those with metal concentrations that were below the upper limits accepted by the European Union for agricultural soils.