Achyranthes bidentata Blume extract protects cultured hippocampal neurons against glutamate-induced neurotoxicity
Achyranthes bidentata Blume extract protects cultured hippocampal neurons against glutamate-induced neurotoxicity
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DOI：
10.1016/j.jep.2009.01.025
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发表时间：
2009-04-21
影响因子：
5.4
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Zhou, Songlin;Chen, Xia;Ding, Fei
我们已经制备了牛膝的水提取物，牛膝是一种常用于关节炎治疗或免疫增强的中草药，并且发现牛膝提取物促进神经生长并防止神经元凋亡。研究目的：研究牛膝提取物对大鼠海马神经元原代培养中谷氨酸诱导的神经毒性的保护作用。材料和方法：采用MTT法检测细胞活力，Hoechst 33342染色和流式细胞术检测细胞凋亡，并采用Fluo 4-AM法、RT-PCR和Western blot法检测细胞内Ca ~（2+）浓度的变化。结果：怀牛膝提取物对谷氨酸诱导的神经元损伤有明显的抑制作用，并呈剂量和时间依赖性。另一方面，牛膝提取物抑制谷氨酸诱导的细胞内钙离子浓度[Ca 2 +]（i）的升高，并在mRNA和蛋白水平上拮抗谷氨酸诱导的Bcl-2表达的降低。结果表明，牛膝提取物通过抑制[Ca 2 +]（i）的增加来防止谷氨酸诱导的原代培养海马神经元细胞损伤，逆转Bcl-2的下调。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
We have prepared an aqueous extract of Achyranthes bidentata Blume, a Chinese medicinal herb commonly prescribed for arthritis treatment or immnopotentiation, and have found that Achyranthes bidentata extract promotes nerve growth and prevents neuronal apoptosis.Aim of the study: To investigate the protective effect of Achyranthes bidentata extract against glutamate-induced neurotoxicity in primary culture of rat hippocampal neurons.Materials and methods: We accomplished MTT assay for cell viability, Hoechst 33342 staining, and flow cytometry for cell apoptosis analysis to examine the effects of Achyranthes bidentata extract on glutamate-induced neurotoxicity, and also used Fluo 4-AM measurement, RT-PCR and Western blot analysis to determine the changes in intracellular calcium concentration [Ca2+](i), and mRNA and protein levels of Bcl-2, respectively, concurrently accompanied with the influences of Achyranthes bidentata extract.Results: Achyranthes bidentata extract was found to inhibit glutamate-induced neuronal damage in a dose- and time-dependent manner. On the other hand, Achyranthes bidentata extract depressed glutamate-induced elevation of intracellular calcium concentration [Ca2+](i), and also antagonized glutamate-evoked decreases in Bcl-2 expression at mRNA and protein levels.Conclusion: The results suggest that Achyranthes bidentata extract prevents glutamate-induced cell damage in primarily cultured hippocampal neurons by inhibiting an increase in [Ca2+](i), and reversing the down-regulation of Bcl-2. (C) 2009 Elsevier Ireland Ltd. All rights reserved.