Cutting edge: Peripheral neuropeptides attract immature and arrest mature blood-derived dendritic cells
Cutting edge: Peripheral neuropeptides attract immature and arrest mature blood-derived dendritic cells
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DOI：
10.4049/jimmunol.166.4.2167
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发表时间：
2001-02-15
影响因子：
4.4
通讯作者：
Wiedermann, CJ
中科院分区：
文献类型：
--
作者：
Dunzendorfer, S;Kaser, A;Wiedermann, CJ
树突状细胞（DC）是一种高度运动的细胞，在多种组织和淋巴器官中介导免疫应答中起着关键作用。我们研究了运动的单核细胞来源的DC在不同成熟阶段的梯度感觉神经肽在体外。降钙素基因相关肽、血管活性肠多肽、分泌素和分泌神经素诱导的未成熟DC趋化性与RANTES的效力相当，而P物质和巨噬细胞炎性蛋白-3 β仅轻微刺激未成熟细胞迁移，生物标记板分析揭示了神经肽诱导的真实趋化反应。巨噬细胞炎性蛋白3 β和6 C因子诱导的细胞迁移。成熟依赖的迁移行为的变化与DC中不同的神经肽诱导的信号转导相一致。外周神经肽可以引导未成熟的DC进入外周神经纤维，在那里高浓度的这些肽可以阻止同时成熟的细胞，感觉神经的功能之一似乎是将DC固定在炎症部位。
Dendritic cells (DC) are highly motile and play a key rot in mediating immune responses in various tissues and lymphatic organs. We investigated locomotion of mononuclear cell-derived DC at different maturation stages toward gradients of sensory neuropeptides in vitro. Calcitonin gene-related peptide, vasoactive intestinal polypeptide, secretin, and secretoneurin induced immature DC chemotaxis comparable to the potency of RANTES, whereas substance P and macrophage-inflammatory protein-3 beta stimulated immature cell migration only slightly, Checkerboard analyses revealed a true chemotactic response induced by neuropeptides, Upon maturation of DC, neuropeptides inhibited spontaneous, macrophage-inflammatory protein-3 beta- and 6Ckine-induced cell migration. Maturation-dependent changes in migratory behavior coincided with distinct neuropeptide-induced signal transduction in DC. Peripheral neuropeptides might guide immature DC to peripheral nerve fibers where high concentrations of these peptides can arrest the meanwhile matured cells, It seems that one function of sensory nerves is to fasten DC at sites of inflammation.