Derivation of male germ cells from bone marrow stem cells
Derivation of male germ cells from bone marrow stem cells
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DOI：
10.1038/labinvest.3700429
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发表时间：
2006-07-01
影响因子：
5
通讯作者：
Engel, Wolfgang
中科院分区：
文献类型：
--
作者：
Nayernia, Karim;Lee, Jae Ho;Engel, Wolfgang
最近的研究表明，无论是植入组织还是在不同条件下培养，体细胞都具有比预期更灵活的潜力。骨髓来源的干细胞可以转分化为多系细胞，如中胚层的肌肉、内胚层的肺和肝，以及外胚层的脑和皮肤。我们在这里展示了骨髓干细胞能够转分化为雄性生殖细胞。为了从成年骨髓干细胞(BMS)中获得雄性生殖细胞，我们使用了在雄性生殖细胞中特异表达绿色荧光蛋白(EGFP)的Stra8增强型绿色荧光蛋白(EGFP)转基因小鼠。BMS细胞来源的生殖细胞表达已知的原始生殖细胞的分子标记，如Fragilis、Stella、RNF17、MVH和Oct4，以及精原干细胞和精原细胞的分子标记，包括RBM、c-Kit、Tex18、Stra8、PIWIL2、DAZL、Hsp90α、β1和α6-整合素。我们从BMS细胞中提取雄性生殖细胞的能力揭示了生殖细胞发育的新方面，并为这些细胞在生殖医学中的使用打开了可能性。
Recent studies have demonstrated that somatic stem cells have a more flexible potential than expected, whether put into tissue or cultured under different conditions. Bone marrow (BM)-derived stem cells can transdifferentiate into multilineage cells, such as muscle of mesoderm, lung and liver of endoderm, and brain and skin of ectoderm origin. Here we show that BM stem cells are able to transdifferentiate into male germ cells. For derivation of male germ cells from adult BM stem (BMS) cells, we used the Stra8-enhanced green fluoresence protein (EGFP) transgenic mouse line expressing EGFP specifically in male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells, such as fragilis, stella, Rnf17, Mvh and Oct4; as well as molecular markers of spermatogonial stem cells and spermatogonia including Rbm, c-Kit, Tex18, Stra8, Piwil2, Dazl, Hsp90 alpha, beta 1- and alpha 6-integrins. Our ability to derive male germ cells from BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine.