Yeast contain a non-proteinaceous pool of copper in the mitochondrial matrix
Yeast contain a non-proteinaceous pool of copper in the mitochondrial matrix
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DOI：
10.1074/jbc.m312693200
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发表时间：
2004-04-02
影响因子：
4.8
通讯作者：
Winge, DR
中科院分区：
文献类型：
--
作者：
Cobine, PA;Ojeda, LD;Winge, DR
酵母菌胞内含有一个铜池，与两个已知的线粒体铜酶，超氧化物歧化酶（SOD 1）和细胞色素c氧化酶（CCO）和铜结合CCO组装蛋白Cox11，Cox17和Sco 1不同。只有一小部分线粒体铜与这些铜蛋白有关。剩余部分的大部分作为可溶性、阴离子、低分子量复合物定位在基质内。基质铜配体的身份是未知的，但大部分的基质铜部分是不结合蛋白质。线粒体铜库是动态的，响应于细胞溶质铜水平的变化。向生长培养基中添加铜盐导致线粒体铜的增加，但该基质池的膨胀不会引起任何呼吸缺陷。基质铜池可接近异源铜酶。线粒体基质中人Sod 1和金属伴侣CCS的共定位导致Sod 2 Delta细胞生长缺陷的抑制。然而，在基质中不存在CCS的情况下，可以通过向生长培养基中添加铜盐来实现人Sod 1的活化。
The yeast mitochondrion is shown to contain a pool of copper that is distinct from that associated with the two known mitochondrial cuproenzymes, superoxide dismutase (Sod1) and cytochrome c oxidase (CcO) and the copper-binding CcO assembly proteins Cox11, Cox17, and Sco1. Only a small fraction of mitochondrial copper is associated with these cuproproteins. The bulk of the remainder is localized within the matrix as a soluble, anionic, low molecular weight complex. The identity of the matrix copper ligand is unknown, but the bulk of the matrix copper fraction is not protein-bound. The mitochondrial copper pool is dynamic, responding to changes in the cytosolic copper level. The addition of copper salts to the growth medium leads to an increase in mitochondrial copper, yet the expansion of this matrix pool does not induce any respiration defects. The matrix copper pool is accessible to a heterologous cuproenzyme. Co-localization of human Sod1 and the metallochaperone CCS within the mitochondrial matrix results in suppression of growth defects of sod2Delta cells. However, in the absence of CCS within the matrix, the activation of human Sod1 can be achieved by the addition of copper salts to the growth medium.