Multiple phase variation in haemolytic. Adhesive and antigenic properties of Streptococcus gordonii
Multiple phase variation in haemolytic. Adhesive and antigenic properties of Streptococcus gordonii
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DOI：
10.1099/13500872-142-1-181
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发表时间：
1996-01-01
期刊：
影响因子：
2.8
通讯作者：
Vickerman, MM
中科院分区：
文献类型：
--
作者：
Jones, GW;Clewell, DB;Vickerman, MM
戈登链球菌在含有洗涤过的马红细胞的琼脂培养基上产生β-溶血性变体（β-溶血素产生的Bhp（+）），频率为10（-4）-10（-3）。Bhp（+）变异体以相同的频率恢复为野生型α-溶血性表型（Bhp（-）），Bhp的相位变异和先前描述的Spp（蔗糖促进表型）的相位变异将同时发生，但这些表型之间不存在相关性。也有证据表明，在唾液包被的羟基磷灰石（粘附到唾液膜的天冬氨酸），在乳糖敏感的共聚集（共聚集，乳糖敏感的Cls）和特定的细胞表面抗原的浓度（帽细胞抗原谱）在菌株中经历了阶段的变化，在Spp和/或Bhp的粘附独立的阶段变化。在所有这些表型的阶段变化之间的高和低水平的活动和每一个似乎发生作为一个独立的事件的过渡。然而，Bhp和Asp等表型之间以及Asp、Cls和Cap表型之间的显著关联（列联表分析P <0.0001）是明显的。Gordonii细胞变得易于发生相变化，并且所产生的独立表型变化可能产生表型多样的链球菌群体，其能够适应口腔中快速和短暂的环境变化。
Streptococcus gordonii gave rise to beta-haemolytic variants (Bhp(+) for beta-haemolysin production) at frequencies of 10(-4)-10(-3) on agar medium containing washed horse erythrocytes. Bhp(+) variants reverted to the wild-type alpha-haemolytic phenotype (Bhp(-)) at the same frequencies, There was a significant probability (greater than or equal to 0.1) that phase variation in Bhp and phase variation in the previously described Spp (sucrose promoted phenotype) would occur concomitantly, but there was no correlation between these phenotypes. There was evidence also of independent phase variation in adhesion to saliva-coated hydroxyapatite (Asp for adhesion to salivary pellicles), in lactose-sensitive coaggregation (Cls for coaggregation, lactose-sensitive) and in the concentrations of particular cell surface antigens (Cap for cell antigen profile) in strains that had undergone phase changes in Spp and/or Bhp. Phase variation in all these phenotypes were transitions between high and low levels of activity and each appeared to occur as an independent event. Significant associations (P much less than 0.0001 by contingency table analysis) between particular phenotypes such as Bhp and Asp and between Asp, Cls and Cap phenotypes, however, were apparent, The results suggest that S. gordonii cells become predisposed to phase variation and that the resulting independent phenotypic changes may give rise to phenotypically diverse streptococcal populations able to accommodate rapid and transient environmental changes in the mouth.