ACTIVATION OF PRIMARY PORCINE ENDOTHELIAL CELLS INDUCES RELEASE OF PORCINE ENDOGENOUS RETROVIRUSES
ACTIVATION OF PRIMARY PORCINE ENDOTHELIAL CELLS INDUCES RELEASE OF PORCINE ENDOGENOUS RETROVIRUSES
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原代猪内皮细胞的激活诱导猪内源性逆转录病毒的释放
DOI：
10.1097/01.tp.0000114966.20491.50
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发表时间：
2004
期刊：
影响因子：
6.2
通讯作者：
G. Langford
中科院分区：
文献类型：
--
作者：
D. Cunningham;G. J. Dos Santos Cruz;X. M. Fernández;A. Whittam;C. Herring;L. Copeman;A. Richards;G. Langford
背景。内皮细胞形成猪移植物和受体之间的界面，在异种移植后经常被激活。为了进一步评估异种移植的安全性，我们评估了细胞活化对从转基因和非转基因猪分离的原代内皮细胞中表达和释放猪内源性逆转录病毒的影响。方法。用人肿瘤坏死因子-&agr处理原代猪内皮细胞；猪干扰素γ或脂多糖。采用基于聚合酶链反应的逆转录酶（PBRT）检测，每隔24小时（最多72小时）监测猪内源性逆转录病毒向上清液中的释放情况。活化和未活化的内皮细胞与人细胞共培养，以研究从猪细胞释放的任何病毒感染人细胞的能力。结果。通过PBRT分析，静止细胞的上清液中未检测到病毒。肿瘤坏死因子-&agr活化细胞后，内皮细胞释放的病毒颗粒数为103 ~ 5×105病毒颗粒/mL；如PBRT分析所示，干扰素-γ或脂多糖。相比之下，体外感染的人细胞只有未活化的内皮细胞，没有观察到与活化的猪细胞共培养。结论。细胞因子处理原代猪内皮细胞导致病毒释放到上清液中增加，但观察到的病毒滴度增加并没有反映在对人类细胞的感染性增加上。
Background. Endothelial cells form the interface between the porcine graft and the recipient and frequently become activated after xenotransplantation. To evaluate the safety of xenotransplantation further, we assessed the effect of cellular activation on the expression and release of porcine endogenous retroviruses from primary endothelial cells isolated from transgenic and nontransgenic pigs. Methods. Primary porcine endothelial cells, cultured from pigs transgenic for human decay accelerating factor, were treated with human tumor necrosis factor-&agr;, porcine interferon-γ, or lipopolysaccharide. The release of porcine endogenous retroviruses into the supernatant was monitored at 24-hr intervals (up to 72 hr) by polymerase chain reaction-based reverse transcriptase (PBRT) assay. Activated and unactivated endothelial cells were co-cultured with human cells to investigate the capacity of any virus released from the porcine cells to infect human cells. Results. Virus was not detected in supernatants from quiescent cells by PBRT analysis. The number of viral particles released from endothelial cells was 103 to 5×105 viral particles/mL after cellular activation with tumor necrosis factor-&agr;, interferon-γ, or lipopolysaccharide, as shown by PBRT analysis. In contrast, in vitro infection of human cells was observed with unactivated endothelial cells only and was not observed in co-cultures with the activated porcine cells. Conclusions. Cytokine treatment of primary porcine endothelial cells results in an increase in the release of virus into the supernatant, but the observed increase in viral titer was not mirrored by an increase in infectivity toward human cells.
影响因子：
4.4
作者：
Brian R. Lane;D. Markovitz;N. Woodford;Rosemary Rochford;R. Strieter;Michael J. Coffey
通讯作者：
Brian R. Lane;D. Markovitz;N. Woodford;Rosemary Rochford;R. Strieter;Michael J. Coffey
影响因子：
6.2
作者：
Byrne, G;McCurry, KR;Logan, JS
通讯作者：
Logan, JS