GmPIN-dependent polar auxin transport is involved in soybean nodule development
GmPIN-dependent polar auxin transport is involved in soybean nodule development
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DOI：
10.1093/plcell/koab183
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xu Chen
中科院分区：
文献类型：
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作者：
Zhen Gao;Zhiwei Chen;Yuanyuan Cui;Meiyu Ke;Huifang Xu;Qinzhen Xu;Jiaomei Chen;Yang Li;Laimei Huang;Hong Zhao;Dingquan Huang;Siyuan Mai;Tao Xu;Xiao Liu;Yuefeng Guan;Wenqiang Yang;Jiří Friml;Jan Petrášek;Jing Zhang;Xu Chen
To overcome nitrogen deficiency, legume roots establish symbiotic interactions with nitrogen-fixing rhizobia that are fostered in specialized organs (nodules). Similar to other organs, nodule formation is determined by a local maximum of the phytohormone auxin at the primordium site. However, how auxin regulates nodule development remains poorly understood. Here, we found that in soybean, (Glycine max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is involved in nodule primordium formation. GmPIN1 was specifically expressed in nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated with CRISPR-Cas9 showed the impaired establishment of auxin maxima in nodule meristems and aberrant divisions in the nodule primordium cells. Moreover, overexpression of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement. Our findings reveal how PIN-dependent auxin transport modulates different aspects of soybean nodule development and suggest that the establishment of auxin gradient is a prerequisite for the proper interaction between legumes and rhizobia.
