[Histopathology of lung of rats exposed to suspension of PM(2.5) with silver staining].
[Histopathology of lung of rats exposed to suspension of PM(2.5) with silver staining].
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DOI：
10.3760/cma.j.issn.1001-9391.2007.09.007
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发表时间：
2007-09
影响因子：
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通讯作者：
Xu Yang;Chang-li Yue;Qin Wang;Ning-hua Huang;Dongqun Xu;Hong-Gang Liu
中科院分区：
文献类型：
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作者：
Xu Yang;Chang-li Yue;Qin Wang;Ning-hua Huang;Dongqun Xu;Hong-Gang Liu
目的观察PM（2.5）悬浮液对大鼠肺组织病理学的影响，并检测银染色显示肺内粉尘颗粒沉积的影响。方法收集北京市空气中游离性PM(2.5)，制作悬浮液。将大鼠分为不同组，每周气管内注射不同剂量的PM(2.5) （0.3 mg/0.2 ml /只大鼠、0.75 mg/0.2 ml /只大鼠、2 mg/0.2 ml /只大鼠）。治疗后4周、12周、24周（总剂量：7.2 mg /只、18 mg /只、48 mg /只）处死，取肺标本。采用苏木精伊红（HE）染色、Warthin-Starry （WS）银染色、透射电镜（TEM）观察大鼠肺组织病理变化及肺组织情况。用x射线光谱化学元素分析（X-RSA）对肺内粉尘颗粒进行了观察。对大鼠肺内肉芽肿病变进行计数，测定肺内沉积尘埃颗粒的沉积程度、积光密度（IOD）值和积面积密度（IAD）值。方差、最小显著性差异、一元线性相关及回归分析。结果大鼠肺内肉芽肿病变数量随时间增加而增多。在WS染色中，尘埃颗粒呈深棕色并变得更清晰。这些尘埃颗粒的IOD和IAD值在WS染色下明显高于HE染色（P < 0.05）。尘埃颗粒的IOD值与肉芽肿病变数呈正相关（R = 0.639, P < 0.01）。透射电镜观察大鼠肺内粉尘颗粒与游离性PM（2.5）超微结构基本一致。通过X-RSA，它们的主要成分相似，都是硅。结论PM（2.5）悬浮可引起大鼠肺部肉芽肿病变。WS银染色较好地显示PM（2.5）被巨噬细胞吞噬，且优于HE染色。
OBJECTIVE To observe the histopathological changes of the lung of rats which exposed to the suspension of PM(2.5) and detect the effect of silver staining showing dust particles deposited in the lungs. METHODS The dissociative PM(2.5) of Beijing city was collected to make suspension. The rats were divided into different groups and exposed to different dosage of PM(2.5) (0.3 mg/0.2 ml per rat, 0.75 mg/0.2 ml per rat, 2 mg/0.2 ml per rat) by intratracheal instillation every week. These rats were sacrificed at 4, 12 weeks and 24 weeks (total dosage: 7.2 mg per rat, 18 mg per rat, 48 mg per rat) after the treatment, and their lungs were sampled. The pathological varieties and the situation of these rats' lungs were observed macroscopically and using hematoxylin and eosin (HE) staining, Warthin-Starry (WS) silver stain, as well as the transmission electron microscope (TEM). The dust particles in these rats' lungs were observed by x-ray spectrum chemical element analysis (X-RSA). The granulomatous lesion in the lungs of the rats was counted, and the deposition degree, integrated optical density (IOD) value and integrated area density (IAD) value of the dust particles deposited in the lungs were measured. The variance, least significance difference, and the unitary linear related and regression were analyzed. RESULTS The number of the granulomatous lesion in the lungs of the rats became more and more with time. In WS staining the dust particles were dark brown and became clearer. The IOD and IAD value of these dust particles were much higher in WS staining than that in HE staining (P < 0.05). The IOD value of the dust particles was positively correlated with the number of the granulomatous lesion (R = 0.639, P < 0.01). The ultrastructure of the dust particles in the rats' lungs and the dissociative PM(2.5) was basically same in TEM. Their main compositions were similar, by X-RSA, and both of them were silicon. CONCLUSION The suspension of PM(2.5) could result in the granulomatous lesion in the lung of rats. WS silver staining is a good method to show PM(2.5) phagocytized by macrophage, and is better than HE staining.