Sex determination in hydra: control by a subpopulation of interstitial cells in Hydra oligactis males.
Sex determination in hydra: control by a subpopulation of interstitial cells in Hydra oligactis males.
复制标题
水螅的性别决定：由雄性少水螅中的间质细胞亚群控制。
DOI：
10.1016/0012-1606(86)90311-8
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发表时间：
1986
影响因子：
2.7
通讯作者：
Littlefield,CL
中科院分区：
文献类型：
--
作者：
Littlefield,CL
在18 ℃和22 ℃两种培养温度下，对寡聚水螅（Hydra oligactismales）的单克隆进行了有性表型稳定性的研究。在这些温度下，本物种的动物不进行有性繁殖，而是通过出芽进行无性繁殖，雄性和雌性在形态上难以区分。当温度降低到10°C时，配子发生被诱导，并且可以测定性表型。在18°C下培养数年的雄性在被诱导进行配子发生时表现出稳定的性表型;雄性仍然是雄性。然而，那些在22°C下培养1年的细胞表现出从雄性到雌性的性反转频率很低;雄性停止精子分化并开始产生卵子。在较高温度下培养的雄性性别逆转与间质细胞的特定亚群的丢失相关，这些细胞与单克隆抗体AC 2结合，AC 2标记H中生精途径特异性的细胞。寡头政治当限制于该途径的间质细胞被重新引入性别逆转的雄性（表型雌性）时，雄性表型被重新建立，动物恢复到精子生产。为了进一步研究AC 2+细胞在雌性雄性化中的作用，将正常雄性（含有AC 2+细胞）和性别逆转的雄性（缺乏AC 2+细胞）移植到雌性。在正常的男性和女性之间的移植，卵子生产停止和精子分化随之而来，而性别逆转的男性和女性之间的移植继续产生卵子。因此，AC 2+间质细胞的存在与雄性性表型严格相关，只有在它们不存在时，雌性表型才表达。
The stability of sexual phenotype was examined in a single clone ofHydra oligactismales maintained at two culture temperatures, 18 and 22°C. At these temperatures animals of this species do not reproduce sexually, but reproduce asexually by budding, and males and females are morphologically indistinguishable. When the temperature is lowered to 10°C gametogenesis is induced and sexual phenotype can be assayed. Males cultured for several years at 18°C expressed a stable sexual phenotype when induced to undergo gametogenesis; males remained male. Those cultured at 22°C for 1 year, however, expressed a low frequency of sex reversal from male to female; males ceased sperm differentiation and began producing eggs. Male sex reversal in cultures maintained at the higher temperature was correlated with the loss of a specific subpopulation of interstitial cells, those that bind the monoclonal antibody, AC2, which labels cells specific to the spermatogenic pathway inH. oligactismales. When interstitial cells restricted to this pathway were reintroduced into sex-reversed males (phenotypic females), the male phenotype was reestablished and animals reverted to sperm production. To further investigate the role of AC2+ cells in the masculinization of females, normal males (containing AC2+ cells) and sex-reversed males (lacking AC2+ cells) were grafted to females. In grafts between normal males and females, egg production ceased and sperm differentiation ensued, whereas those between sex-reversed males and females continued to produce eggs. Thus, the presence of AC2+ interstitial cells is strictly correlated with male sexual phenotypes and it is only in their absence that the female phenotype is expressed.