Colocalization of emerin and lamins in interphase nuclei and changes during mitosis
Colocalization of emerin and lamins in interphase nuclei and changes during mitosis
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DOI：
10.1006/bbrc.1998.9209
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发表时间：
1998-08-28
影响因子：
3.1
通讯作者：
Morris, GE
中科院分区：
文献类型：
--
作者：
Manilal, S;Man, NT;Morris, GE
Emerin是一种核膜蛋白，在X连锁Emery-Dreifuss肌营养不良症中受到突变的影响。我们先前认为Emerin是与核膜相关的II型完整膜蛋白家族的成员，该家族包括膜相关蛋白和层蛋白B受体。我们现在发现，在COS细胞中，Emerin不仅限于核缘，还存在于核内部位，在那里它与核层蛋白B1、B2和A/C共存。在有丝分裂过程中，Emerin分散在整个细胞中，然后参与子核周围膜的重建。虽然Emerin和Lamins在有丝分裂过程中并不保持共存，但它们都与有丝分裂纺锤体的中体有一定的联系。(C)1998年学术出版社。
Emerin is a nuclear membrane protein which is affected by mutation in X-linked Emery-Dreifuss muscular dystrophy. We have previously suggested that emerin is a member of a family of type II integral membrane proteins which associate with the nuclear lamina and which include lamina-associated proteins and the lamin B receptor. We now show that emerin in COS cells is not restricted to the nuclear rim but is also found at intranuclear sites, where it colocalizes with nuclear lamins B1, B2 and A/C. During mitosis, emerin is dispersed throughout the cell and then participates in the reconstitution of membranes around the daughter nuclei. Although emerin and lamins do not remain colocalized during mitosis, they all show some association with the midbody of the mitotic spindle. (C) 1998 Academic Press.