Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
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DOI：
10.1016/j.jbiotec.2006.02.021
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发表时间：
2006-09-01
影响因子：
4.1
通讯作者：
Saddler, Jack
中科院分区：
文献类型：
--
作者：
Berlin, Alex;Balakshin, Mikhail;Saddler, Jack
木质纤维素生物质向燃料乙醇的转化通常涉及破坏性预处理过程，随后是纤维素和hernicellulose组分的酶催化水解为可发酵糖。提高工艺经济性的尝试包括纤维素酶、木聚糖酶和相关水解酶的蛋白质工程化以提高它们的比活性或稳定性。然而，人们认识到，在木质纤维素水解过程中，酶的性能由于与木质素或木质素-碳水化合物复合物（LCC）的相互作用而降低，因此选择或工程化具有降低的木质素相互作用的酶提供了酶改进的替代手段。本研究探讨了抑制7纤维素酶制剂，木聚糖酶制剂和β-葡萄糖苷酶制剂由两个纯化的，颗粒状的木质素制剂来自软木使用有机溶剂预处理工艺，然后通过酶水解。这两种木质素制剂具有相似的粒径和表面积，但在其他物理性质和化学组成方面存在显着差异，所述化学组成由2D相关HSQC NMR技术和定量C-13 NMR光谱确定。不同的纤维素酶在木质素的抑制作用上相差高达3.5倍，而木聚糖酶表现出较小的变异性（
The conversion of lignocellulosic biomass to fuel ethanol typically involves a disruptive pretreatment process followed by enzyme-catalyzed hydrolysis of the cellulose and hernicellulose components to fermentable sugars. Attempts to improve process economics include protein engineering of cellulases, xylanases and related hydrolases to improve their specific activity or stability. However, it is recognized that enzyme performance is reduced during lignocellulose hydrolysis by interaction with lignin or lignin-carbohydrate complex (LCC), so the selection or engineering of enzymes with reduced lignin interaction offers an alternative means of enzyme improvement. This study examines the inhibition of seven cellulase preparations, three xylanase preparations and a beta-glucosidase preparation by two purified, particulate lignin preparations derived from softwood using an organosolv pretreatment process followed by enzymatic hydrolysis. The two lignin preparations had similar particle sizes and surface areas but differed significantly in other physical properties and in their chemical compositions determined by a 2D correlation HSQC NMR technique and quantitative C-13 NMR spectroscopy. The various cellulases differed by up to 3.5-fold in their inhibition by lignin, while the xylanases showed less variability (