NUCLEOTIDE-SEQUENCE OF THE PHOB GENE, THE POSITIVE REGULATORY GENE FOR THE PHOSPHATE REGULON OF ESCHERICHIA-COLI K-12
NUCLEOTIDE-SEQUENCE OF THE PHOB GENE, THE POSITIVE REGULATORY GENE FOR THE PHOSPHATE REGULON OF ESCHERICHIA-COLI K-12
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DOI：
10.1016/0022-2836(86)90073-2
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发表时间：
1986-07-05
影响因子：
5.6
通讯作者：
NAKATA, A
中科院分区：
文献类型：
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作者：
MAKINO, K;SHINAGAWA, H;NAKATA, A
phoB编码属于大肠杆菌磷酸调节子的许多基因的正调节子，包括phoA、phoS、phoE和ugpAB。我们已经确定了含有phoB基因启动子和编码区的染色体片段的核苷酸序列。与已知的PhoB-LacZ杂合蛋白的氨基末端氨基酸序列相结合的序列数据表明，PhoB蛋白由228个氨基酸残基组成，Mr为26，302。在该基因的调控区，发现phoA、phoS和phoE基因调控区共有的一段核苷酸序列，我们将其命名为“磷酸盒”，由于这些基因受phoB产物的正调控，这表明phoB基因的转录也受其自身产物的正调控。在phoB、ompR和dye的产物的氨基酸序列中发现了广泛的同源性，它们都是编码包膜蛋白的许多基因的正调控基因。这意味着这些基因最初是一个原基因的复制品，进化后具有不同但相关的功能。
phoB encodes a positive regulator for a number of the genes belonging to the phosphate regulon of Escherichia coli, including phoA, phoS, phoE and ugpAB. We have determined the nucleotide sequence of the chromosomal segment containing the promoter and the coding region of the phoB gene. The sequence data combined with the known amino-terminal amino acid sequence of a PhoB-LacZ hybrid protein suggest that the PhoB protein consists of 228 amino acid residues with a Mr of 26,302. In the regulatory region of the gene, a consensus nucleotide sequence shared by the regulatory regions of the phoA, phoS and phoE genes, which we name the "phosphate box", was found. Since these genes are positively regulated by the product of phoB, this suggests that transcription of the phoB gene is also regulated positively by its own product. Extensive homology was found in the amino acid sequences of the products of phoB, ompR and dye, all of which are positive regulatory genes for a number of genes coding for envelope proteins. This implies that these genes were originally duplications of a protogene that evolved to have divergent but related functions.