Culture Conditions and Signalling Networks Promoting the Establishment of Cell Lines from Parthenogenetic and Biparental Pig Embryos
Culture Conditions and Signalling Networks Promoting the Establishment of Cell Lines from Parthenogenetic and Biparental Pig Embryos
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DOI：
10.1007/s12015-010-9153-2
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Gandolfi, Fulvio
中科院分区：
文献类型：
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作者：
Brevini, Tiziana A. L.;Pennarossa, Georgia;Gandolfi, Fulvio
猪胚胎干细胞（pESC）的产生将潜在地在生物医学领域产生巨大影响，因为猪作为临床前生物医学应用的主要动物模型的历史悠久。这些细胞也将有利于农业领域，允许对这种动物进行有效的基因工程，以改善健康和生产性状。尽管有许多报道，但在pESC系的分离和繁殖方面尚未获得结论性结果，并且从猪建立多能细胞仍然是一个难以捉摸的目标。在本研究中，我们进行了系统的分析，不同的培养基的能力，以支持建立同质的生长，从体外生产的胚胎。此外，我们研究了哪些分子网络对最有效培养基中所含的因子有反应，因为鉴定调节猪干细胞多能性的显性信号通路可能有助于产生真正的pESC。最后，我们比较了体外受精囊胚与单性生殖细胞作为一个可能的来源，推定pESC的囊胚率，免疫手术程序的弹性，能够附着到饲养，产生生长和建立稳定的细胞系。
The generation of porcine embryonic stem cells (pESC) would potentially have great impact in the biomedical field given the long-standing history of the pig as a prime animal model for pre-clinical biomedical applications. These cells would also be beneficial for the agricultural area, allowing efficient genetic engineering of this animal, to improve health and production traits. Despite numerous reports, no conclusive results have been obtained on the isolation and propagation of pESC lines and the establishment of pluripotent cells from the pig has remained an elusive goal. In the present study we performed a systematic analysis of different culture media for their ability to support the establishment of homogenous out-growths from in vitro-produced embryos. Furthermore, we investigated which molecular networks are responsive to the factors contained in the most efficient media, since the identification of dominant signaling pathways that regulate porcine stem-cell pluripotency is likely to facilitate the generation of genuine pESC. Finally we compared IVF blastocysts versus parthenotes as a possible source for putative pESC in terms of blastocyst rate, resilience to immunosurgery procedures, ability to attach to the feeder, to generate outgrowths and to establish stable cell lines.