Chemical composition, angiotensin I-converting enzyme (ACE) inhibitory, antioxidant and antimicrobial activities of the essential oil from Periploca laevigata root barks
Chemical composition, angiotensin I-converting enzyme (ACE) inhibitory, antioxidant and antimicrobial activities of the essential oil from Periploca laevigata root barks
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DOI：
10.1016/j.foodchem.2010.01.021
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发表时间：
2010-08-01
期刊：
影响因子：
8.8
通讯作者：
Nasri, Moncef
中科院分区：
文献类型：
--
作者：
Hajji, Mohamed;Masmoudi, Ons;Nasri, Moncef
研究了广布于突尼斯的一种芳香植物紫罗兰根皮挥发油的化学成分及其抗菌、抗氧化和血管紧张素转换酶(ACE)抑制活性。用气相色谱/质谱法测定了PLRB油的组成。挥发油中鉴定出43种成分，主要成分为苯甲醛(56%)、4-甲氧基水杨酸甲酯(6.55%)和香芹酚(4.75%)。PLRB挥发油对血管紧张素转换酶的抑制作用呈剂量依赖性。经1，1-二苯基-2-苦皮酰肼(DPPH)自由基法、β-胡萝卜素漂白和还原能力测定表明，PLRB油具有较强的抗氧化活性。同时考察了挥发油对几种微生物的抑菌活性。菌株的抑菌圈为12~46 mm，最小抑菌浓度(MIC)为50~300µg/ml。PLRB挥发油对革兰氏阳性菌的抑制率显著高于对革兰氏阴性菌的抑制率。我们的研究结果表明，莱维果精油可能是进一步研究新的生物活性物质的很好的候选物质。(C)2010爱思唯尔有限公司。保留所有权利。
The present study describes the chemical composition, and antimicrobial, antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of essential oil from Periploca laevigata root barks (PLRB), an aromatic plant widely distributed in Tunisia and used as a traditional medicinal plant. Gas chromatography/mass spectrometry was used to determine the composition of the PLRB oil. Forty-three components were identified in the essential oil and the main compounds were benzaldehyde (56%), methyl 4-methoxysalicylate (6.55%) and carvacrol (4.75%). The PLRB essential oil exhibited a dose-dependent manner of inhibitory activity toward ACE. The highest ACE inhibitory activity (54%) was observed at a concentration of 30 mu g/ml. The PLRB oil was also found to possess antioxidant activities, as evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method, beta-carotene bleaching and reducing power assays. The antimicrobial activity of the essential oil was also investigated on several microorganisms. The inhibition zones and minimal inhibitory concentration (MIC) values of bacterial strains were in the range of 12-46 mm and 50-300 mu g/ml, respectively. The inhibitory activity of the PLRB essential oil against Gram-positive bacteria was significantly higher than against Gram-negative. It also exhibited remarkable activity against several fungal strains.Our findings demonstrate that the essential oil from P. laevigata might be a good candidate for further investigations of new bioactive substances. (C) 2010 Elsevier Ltd. All rights reserved.