Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos.
Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos.
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DOI：
10.1089/cell.2012.0094
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发表时间：
2013-07
影响因子：
1.6
通讯作者：
M. Wittayarat;Yoko Sato;L. Do;Y. Morita;K. Chatdarong;M. Techakumphu;M. Taniguchi;T. Otoi
中科院分区：
文献类型：
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作者：
M. Wittayarat;Yoko Sato;L. Do;Y. Morita;K. Chatdarong;M. Techakumphu;M. Taniguchi;T. Otoi
异常的表观遗传重编程，如组蛋白乙酰化，可能导致异种体细胞核移植的效率低下。本研究旨在探讨曲古抑素A(TSA)对猫体细胞和牛胞质iSCNT重构胚胎发育能力和组蛋白乙酰化的影响。在融合和激活后，用不同浓度的TSA(0、25、50和100 nM)分别处理iSCNT CAT和孤雌牛胚胎24 h。50 nM TSA处理的iSCNT胚胎的卵裂率和囊胚率(分别为84.3%和4.6%)显著高于未处理组(分别为63.8%和0%)。同样，50 nM TSA处理提高了牛孤雌胚胎的囊胚率。经50 nM TSA处理的iSCNT胚胎中组蛋白H3赖氨酸9(H3K9)的乙酰化水平与体外受精胚胎相似，显著高于未经TSA处理的iSCNT胚胎(对照组)，与胚胎发育阶段(2-细胞、4-细胞和8-细胞阶段)无关。这些结果表明，50 nM TSA融合后处理有利于iSCNT猫胚胎发育到囊胚期，并与H3K9乙酰化水平的升高有关。
Abnormal epigenetic reprogramming, such as histone acetylation, might cause low efficiency of interspecies somatic cell nuclear transfer (iSCNT). This study was conducted to evaluate the effects of trichostatin A (TSA) on the developmental competence and histone acetylation of iSCNT embryos reconstructed from cat somatic cells and bovine cytoplasm. The iSCNT cat and parthenogenetic bovine embryos were treated with various concentrations of TSA (0, 25, 50, or 100 nM) for 24 h, respectively, following fusion and activation. Treatment with 50 nM TSA produced significantly higher rates of cleavage and blastocyst formation (84.3% and 4.6%, respectively) of iSCNT embryos than the rates of non-TSA-treated iSCNT embryos (63.8% and 0%, respectively). Similarly, the treatment of 50 nM TSA increased the blastocyst formation rate of parthenogenetic bovine embryos. The acetylation levels of histone H3 lysine 9 (H3K9) in the iSCNT embryos with the treatment of 50 nM TSA were similar to those of in vitro-fertilized embryos and significantly higher (p<0.05) than those of non-TSA-treated iSCNT embryos (control), irrespective of the embryonic development stage (two-cell, four-cell, and eight-cell stages). These results indicated that the treatment of 50 nM TSA postfusion was beneficial for development to the blastocyst stage of iSCNT cat embryos and correlated with the increasing levels of acetylation at H3K9.