Steroid production in vitro by rat ovaries during sexual maturation.
Steroid production in vitro by rat ovaries during sexual maturation.
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性成熟过程中大鼠卵巢体外产生类固醇。
DOI：
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发表时间：
1983
影响因子：
4
通讯作者：
R. van der Linden
中科院分区：
文献类型：
--
作者：
J. Uilenbroek;P. J. Woutersen;R. van der Linden
为了确定性成熟过程中大鼠卵巢类固醇生成的变化，在不存在或存在LH的情况下孵育不同年龄（10 - 35天）和第一次发情前期获得的卵巢，并测量培养基中类固醇的积累。基础和LH刺激的雌二醇-17 β和睾酮释放到培养基中，以pmol/4 h/mg卵巢表示，在10日龄和第一次发情前期较高。在第20和35天之间，基础雌二醇和睾酮的释放较低，并且不能被LH刺激。添加睾酮的培养基中增加雌二醇的生产在所有年龄段的研究。孕酮的释放发生在所有年龄，即使在LH的培养基。在LH的存在下孵育导致孕酮的剂量依赖性增加，在发情前期有最大反应。雄甾酮和5 α-雄甾烷-3 α，17 β-二醇的生产在LH的存在或不存在的情况下是高的，在整个青春期前的时期。从第10天到第20天，5 α还原雄激素对LH的反应增加，但此后减少。同样，5 α-还原酶活性，卵巢匀浆中测量，增加从第10天到第20天，但第一次发情前再次下降。基础和LH刺激的5 α-减少雄激素产生的进一步减少发生在第一次排卵后。这些结果证明了体外孵育后类固醇释放的年龄相关变化。在第10天，孕酮可以转化为可芳香化的雄激素，从而允许雌激素的产生，而在第10天之后，孕酮转化为5 α还原的C19类固醇。（250字处删节）
To determine changes in steroidogenesis by rat ovaries during sexual maturation, ovaries obtained at various ages (days 10-35) and at the first pro-oestrus were incubated in the absence or presence of LH and the accumulation of steroids in the medium was measured. Basal and LH-stimulated oestradiol-17 beta and testosterone release into the medium, expressed in pmol/4 h per mg ovary, was high at day 10 of age and at first pro-oestrus. Between days 20 and 35 basal oestradiol and testosterone release was low and could not be stimulated by LH. Addition of testosterone to the culture medium increased oestradiol production at all ages studied. Release of progesterone occurred at all ages even in LH-free medium. Incubation in the presence of LH resulted in a dose-dependent increase in progesterone with a maximal response at pro-oestrus. Androsterone and 5 alpha-androstane-3 alpha,17 beta-diol production in the absence or presence of LH was high during the entire prepuberal period. Production of 5 alpha-reduced androgens in response to LH increased from days 10 to 20 but decreased thereafter. Similarly, 5 alpha-reductase activity, measured in ovarian homogenates, increased from days 10 to 20 but was decreased again by first pro-oestrus. A further decrease in basal and LH-stimulated 5 alpha-reduced androgen production occurred after first ovulation. These results demonstrated age-related changes in steroid release after in-vitro incubation. At day 10 progesterone can be converted to aromatizable androgens allowing production of oestrogens, while after day 10 progesterone is converted to 5 alpha-reduced C19 steroids.(ABSTRACT TRUNCATED AT 250 WORDS)