Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis.
Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis.
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DOI：
10.1016/j.febslet.2015.09.005
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发表时间：
2015-10-07
期刊：
影响因子：
3.5
通讯作者：
Sontheimer EJ
中科院分区：
文献类型：
--
作者：
Wakefield N;Rajan R;Sontheimer EJ
In many bacteria and archaea, an adaptive immune system (CRISPR-Cas) provides immunity against foreign genetic elements. This system uses CRISPR RNAs (crRNAs) derived from the CRISPR array, along with CRISPR-associated (Cas) proteins, to target foreign nucleic acids. In most CRISPR systems, endonucleolytic processing of crRNA presursors (pre-crRNAs) is essential for the pathway. Here we study the Cas6 endonuclease responsible for crRNA processing in the Type III-A CRISPR-Cas system from Staphylococcus epidermidis RP62a, a model for Type III-A CRISPR-Cas systems, and define substrate requirements for SeCas6 activity. We find that SeCas6 is necessary and sufficient for full-length crRNA biogenesis in vitro, and that it relies on both sequence and stem-loop structure in the 3′ half of the CRISPR repeat for recognition and processing.
