Scoping studies to establish the capability and utility of a real-time bioaerosol sensor to characterise emissions from environmental sources.
Scoping studies to establish the capability and utility of a real-time bioaerosol sensor to characterise emissions from environmental sources.
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DOI：
10.1016/j.scitotenv.2018.08.120
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发表时间：
2019-01
期刊：
影响因子：
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通讯作者：
Z. Nasir;E. Hayes;Ben Williams;T. Gladding;C. Rolph;S. Khera;S. Jackson;A. Bennett;S. Collins-S.-Colli
中科院分区：
文献类型：
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作者：
Z. Nasir;E. Hayes;Ben Williams;T. Gladding;C. Rolph;S. Khera;S. Jackson;A. Bennett;S. Collins-S.-Colli
一种新型的基于双激发波长的生物气溶胶传感器，具有多个荧光波段，称为光谱强度生物气溶胶传感器（SIBS），在五种不同的室外环境中进行了评估。总粒子和荧光粒子的平均浓度变化很大，在农场（分别为2.6 cm−3和0.48 cm−3）和堆肥场（分别为2.32 cm−3和0.46 cm−3）最高，在奶牛场（分别为1.03 cm−3和0.24 cm−3）和污水处理厂（分别为1.03 cm−3和0.25 cm−3）最低。相比之下，与其他站点相比，农业站点的数字加权荧光分数最低（0.18），表明环境源排放的性质和规模具有很高的可变性。荧光发射数据表明，λex 280和λex 370在不同位置的光谱是多模态的，在二次发射峰的波长处强度差异很大。这一发现表明，在这些地点的发射分子组成的差异，可以帮助识别不同环境来源的不同荧光特征。总的来说，这项研究表明，与现有仪器相比，SIBS提供了额外的光谱信息，并且能够解析来自相关生物荧光团的光谱集成信号，可以提高选择性，从而增强对环境来源生物气溶胶的实时表征的辨别和分类策略。然而，需要结合实际研究和改进的数值方法进行详细的实验室测量，以优化和验证与各种大气相关的生物荧光团相关的这些高分辨率光谱特征。
A novel dual excitation wavelength based bioaerosol sensor with multiple fluorescence bands called Spectral Intensity Bioaerosol Sensor (SIBS) has been assessed across five contrasting outdoor environments. The mean concentrations of total and fluorescent particles across the sites were highly variable being the highest at the agricultural farm (2.6 cm−3and 0.48 cm−3, respectively) and the composting site (2.32 cm−3and 0.46 cm−3, respectively) and the lowest at the dairy farm (1.03 cm−3and 0.24 cm−3, respectively) and the sewage treatment works (1.03 cm−3and 0.25 cm−3, respectively). In contrast, the number-weighted fluorescent fraction was lowest at the agricultural site (0.18) in comparison to the other sites indicating high variability in nature and magnitude of emissions from environmental sources. The fluorescence emissions data demonstrated that the spectra at different sites were multimodal with intensity differences largely at wavelengths located in secondary emission peaks for λex 280 and λex 370. This finding suggests differences in the molecular composition of emissions at these sites which can help to identify distinct fluorescence signature of different environmental sources. Overall this study demonstrated that SIBS provides additional spectral information compared to existing instruments and capability to resolve spectrally integrated signals from relevant biological fluorophores could improve selectivity and thus enhance discrimination and classification strategies for real-time characterisation of bioaerosols from environmental sources. However, detailed lab-based measurements in conjunction with real-world studies and improved numerical methods are required to optimise and validate these highly resolved spectral signatures with respect to the diverse atmospherically relevant biological fluorophores.