Endosomes, lysosomes: their implication in gene transfer.
Endosomes, lysosomes: their implication in gene transfer.
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DOI：
10.1016/s0169-409x(99)00066-6
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发表时间：
2000-03
影响因子：
16.1
通讯作者：
R. Wattiaux;Nathanael Laurent;S. Coninck;M. Jadot
中科院分区：
文献类型：
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作者：
R. Wattiaux;Nathanael Laurent;S. Coninck;M. Jadot
裸质粒DNA或与非病毒载体结合的质粒DNA通过胞吞作用被摄取。因此，它必须通过涉及内体和溶酶体的细胞内吞途径。然而，一些DNA分子必须逃离这些细胞器才能到达发生转录的细胞核。在本文中，我们考虑了各种因素可能会影响质粒DNA的运输和影响转染效率。
Plasmid DNA, naked or bound to a non-viral vector, is taken up by endocytosis. As a result, it has to travel through the intracellular endocytic pathway involving endosomes and lysosomes. However, some DNA molecules must escape these organelles to reach the nucleus where transcription takes place. In this paper, we consider different factors that could affect the trafficking of plasmid DNA and influence transfection efficiency.