Similar Roles of Substantia Nigra and Ventral Tegmental Dopamine Neurons in Reward and Aversion
Similar Roles of Substantia Nigra and Ventral Tegmental Dopamine Neurons in Reward and Aversion
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DOI：
10.1523/jneurosci.1703-13.2014
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发表时间：
2014-01-15
影响因子：
5.3
通讯作者：
Ikemoto, Satoshi
中科院分区：
文献类型：
--
作者：
Ilango, Anton;Kesner, Andrew J.;Ikemoto, Satoshi
腹侧被盖区（VTA）的多巴胺神经元与情感功能有关。然而，黑质致密部（SNc）的多巴胺神经元在多大程度上发挥此类作用尚不清楚。TH - Cre转基因小鼠在VTA或SNc中接受编码视紫红质通道蛋白2（ChR2）、盐细菌视紫红质（NpHR）或对照载体的腺相关病毒载体，导致这些视蛋白在多巴胺神经元中选择性表达。携带ChR2的小鼠学会了通过操作性反应将光刺激传递到VTA或SNc，并且还会寻找它们接受光刺激的区域（即操作性位置偏好）。操作性位置偏好分数与自我刺激反应高度相关。相比之下，携带NpHR的小鼠会避开在VTA、SNc或背侧纹状体接受光刺激的区域，而对照小鼠则不会。这些观察结果表明，SNc多巴胺神经元的兴奋和抑制分别引发积极和消极的情感效应，这与VTA多巴胺神经元的情况相似。
Dopamine neurons in the ventral tegmental area (VTA) are implicated in affective functions. However, it is unclear to what extent dopamine neurons in substantia nigra pars compacta (SNc) play such roles. TH-Cre transgenic mice received adeno-associated viral vectors encoding channelrhodopsin2 (ChR2), halorhodopsin (NpHR), or control vector into the VTA or SNc, resulting in selective expression of these opsins in dopamine neurons. Mice with ChR2 learned instrumental responding to deliver photostimulation into the VTA or SNc and also sought for the compartment where they received photostimulation (i.e., operant place preference). Operant place preference scores were highly correlated with self-stimulation responses. In contrast, mice with NpHR avoided the compartment where they received photostimulation into the VTA, SNc, or dorsal striatum, whereas control mice did not. These observations suggest that the excitation and inhibition of SNc dopamine neurons elicit positive and negative affective effects, respectively, similar to those of VTA dopamine neurons.