Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall
Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall
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DOI：
10.1016/j.biomaterials.2004.04.034
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发表时间：
2005-04-01
期刊：
影响因子：
14
通讯作者：
Vyavahare, NR
中科院分区：
文献类型：
--
作者：
Isenburg, JC;Simionescu, DT;Vyavahare, NR
戊二醛（Glut）固定组织在心血管手术中的进行性变性和钙化限制了其长期临床表现。这种有限的生物稳定性可能是由于Glut不能充分保护某些组织成分，如弹性蛋白免受酶的攻击。我们研究的目的是通过使用单宁酸（TA），一种能够保护弹性蛋白不被特定酶消化的植物多酚，开发专门针对弹性蛋白稳定的新型组织处理技术。在目前的研究中，我们证明了Glut不能充分保护猪主动脉免受体外弹性酶介导的降解。在Glut固定过程中加入TA，使Glut固定主动脉对弹性酶消化的稳定性提高了15倍，并使大鼠皮下模型的钙化降低了66%。TA发现与Glut固定物在化学上是相容的，并且在热变性温度、抗胶原酶和机械性能的微小变化中没有发现胶原交联。体外和体内研究也表明，TA与主动脉壁的结合在很长一段时间内是稳定的。谷氨酸固定心血管植入物中ta介导的弹性蛋白稳定可以显著延长这些组织替代物的临床耐久性。(C) 2004 Elsevier Ltd.版权所有。
Progressive degeneration and calcification of glutaraldehyde (Glut) fixed tissues used in cardiovascular surgery restrict their long-term clinical performance. This limited biological stability may be attributable to the inability of Glut to adequately protect certain tissue components such as elastin from enzymatic attack. The aim of our studies was to develop novel tissue-processing techniques targeted specifically at elastin stabilization by using tannic acid (TA), a plant polyphenol capable of protecting elastin from digestion by specific enzymes. lit present studies we demonstrated that Glut does not adequately protect porcine aorta from elastase-mediated degradation in vitro. The addition of TA to the Glut fixation process increased the stability of Glut-fixed aorta to elastase digestion by 15-fold and also decreased calcification in the rat subdermal model by 66%. TA was found to be chemically compatible with Glut fixation and did not Finder collagen crosslinking as shown by minor changes in thermal denaturation temperatures, resistance to collagenase and mechanical properties. In vitro and in vivo studies also revealed that TA binding to aortic wall was stable over an extended period of time. TA-mediated elastin stabilization in Glut-fixed cardiovascular implants may significantly extend the clinical durability of these tissue replacements. (C) 2004 Elsevier Ltd. All rights reserved.