Endogenous levels of gibberellins, IAA and cytokinins in tobacco crown gall tissues of different morphologies
Endogenous levels of gibberellins, IAA and cytokinins in tobacco crown gall tissues of different morphologies
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不同形态烟草冠瘿组织中赤霉素、IAA和细胞分裂素的内源水平
DOI：
10.1093/oxfordjournals.pcp.a077319
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发表时间：
1987
影响因子：
4.9
通讯作者：
K. Syōno
中科院分区：
文献类型：
--
作者：
S. Nakagawa;D. Tjokrokusumo;A. Sakurai;I. Yamaguchi;N. Takahashi;K. Syōno
采用GC-SIM（GA和细胞分裂素）或带荧光检测器的HPLC（IAA）检测烟草畸胎瘤和无组织冠瘿组织中赤霉素（GA）、IAA和细胞分裂素的内源水平。用章鱼碱型和胭脂碱型Ti质粒诱导两种不同类型的冠瘿。在两种类型中，芽形成畸胎瘤中GA含量高于无组织愈伤组织，而IAA含量高于无组织愈伤组织。而在畸胎瘤中，章鱼碱型细胞的细胞分裂素含量高于畸胎瘤，而在畸胎瘤中，胭脂碱型细胞的细胞分裂素含量高于畸胎瘤。我们的研究结果表明，细胞分裂素/IAA平衡与烟草冠瘿形态之间并不总是有关系，但GA的产生在烟草组织中的分化密切相关。
Endogenous levels of gibberellin (GA) as well as IAA and cytokinins in teratomas and unorganized crown gall tissues of tobacco were examined by GC-SIM (for GA and cytokinin) or HPLC with a fluorescence detector (for IAA). Two different types of crown gall induced by octopine-type and nopaline-type Ti-plasmids were used. In both types, GA contents were higher in shoot-forming teratomas than in unorganized calluses, while IAA contents were higher in unorganized calluses. But cytokinin contents in octopine-type cells were higher in unorganized calluses than in teratomas, whereas the contents in nopaline-type cells were higher in teratomas. Our results suggest that there is not always a relationship between the cytokinin/IAA balance and tobacco crown gall morphology, but GA production in tobacco tissues is closely related to its differentiation.