Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish.
Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish.
复制标题
DOI：
10.1007/978-1-0716-0830-2_13
复制
发表时间：
2021
影响因子：
--
通讯作者：
Y. Ozdemir;C. A. Hansen;Mohamed Ramy;Eileen L. Troconis;Lauren D McNeil;Josef G. Trapani
中科院分区：
文献类型：
--
作者：
Y. Ozdemir;C. A. Hansen;Mohamed Ramy;Eileen L. Troconis;Lauren D McNeil;Josef G. Trapani
斑马鱼是研究脊椎动物感觉编码和行为的许多方面的优秀模式生物。它们的逃避反应开始于C形的身体弯曲，然后是几轮游泳，远离潜在的威胁刺激。这种高度刻板印象的运动行为为研究惊吓反射和多感觉编码和运动的神经回路提供了一个模型。通道视紫红质(ChR2)可在斑马鱼的侧线和耳毛细胞中表达，并可在体内被激发以引发这些快速逃逸形式。在这里，我们综述了我们研究转基因表达ChR2斑马鱼幼虫的方法，包括筛选ChR2的阳性表达，记录场电位和高速视频光学诱发的逃逸反应。我们还强调了所获得数据的重要特征，并简要回顾了其他利用或有可能受益于ChR2和光遗传学的斑马鱼研究。
Zebrafish are an excellent model organism to study many aspects of vertebrate sensory encoding and behavior. Their escape responses begin with a C-shaped body bend followed by several swimming bouts away from the potentially threatening stimulus. This highly stereotyped motor behavior provides a model for studying startle reflexes and the neural circuitry underlying multisensory encoding and locomotion. Channelrhodopsin (ChR2) can be expressed in the lateral line and ear hair cells of zebrafish and can be excited in vivo to elicit these rapid forms of escape. Here we review our methods for studying transgenic ChR2-expressing zebrafish larvae, including screening for positive expression of ChR2 and recording field potentials and high-speed videos of optically evoked escape responses. We also highlight important features of the acquired data and provide a brief review of other zebrafish research that utilizes or has the potential to benefit from ChR2 and optogenetics.