Fundamentals of protein interaction network mapping.
Fundamentals of protein interaction network mapping.
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DOI：
10.15252/msb.20156351
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发表时间：
2015-12-17
影响因子：
9.9
通讯作者：
Stagljar I
中科院分区：
文献类型：
--
作者：
Snider J;Kotlyar M;Saraon P;Yao Z;Jurisica I;Stagljar I
研究生物体中所有蛋白质的蛋白质相互作用网络（“相互作用组”）仍然是现代生物医学的主要挑战之一。这些信息对于理解细胞通路和开发治疗人类疾病的有效疗法至关重要。在过去的二十年中，已经开发了多种生物化学、遗传学和细胞生物学方法来绘制相互作用组。在这篇综述中，我们强调相互作用基因组定位的基本原则。具体而言，我们讨论了单个检测的优点和缺点，如何选择适合所研究问题的方法，并为进行必要的后续分析提供一般指导。此外，我们还讨论了预测、绘制和可视化相互作用组的计算方法，并提供了一些最重要的相互作用组资源的总结。我们希望这篇综述既能作为该领域的有用概述，也能作为指导，帮助更多的科学家在他们的研究中积极采用这些强大的方法。
Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the past two decades, diverse biochemical, genetic, and cell biological methods have been developed to map interactomes. In this review, we highlight basic principles of interactome mapping. Specifically, we discuss the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and provide general guidelines for carrying out the necessary follow‐up analyses. In addition, we discuss computational methods to predict, map, and visualize interactomes, and provide a summary of some of the most important interactome resources. We hope that this review serves as both a useful overview of the field and a guide to help more scientists actively employ these powerful approaches in their research.