Cells expressing GABA synthetic enzyme, glutamate decarboxylase, in stomach and intestine: RT-PCR and immunohistochemistry studies
Cells expressing GABA synthetic enzyme, glutamate decarboxylase, in stomach and intestine: RT-PCR and immunohistochemistry studies
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胃和肠中表达 GABA 合成酶、谷氨酸脱羧酶的细胞：RT-PCR 和免疫组织化学研究
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
H. Ueno
中科院分区：
文献类型：
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作者：
K. Akamatsu;Yumi Nakamura;H. Hayasaki;Kiyohito Kanabara;K. Maemura;Y. Yanagawa;K. Obata;Masahito Watanabe;H. Ueno
应用RT-PCR和免疫组织化学技术研究了小鼠胃、肠组织中谷氨酸脱羧酶的表达。RT-PCR研究显示GAD mRNA在4周龄和11周龄小鼠的胎龄和生后年龄中表达。在4周龄和11周龄小鼠的胃和肠中均观察到针对GAD 65的强阳性染色。在胎儿的胃和肠中均发现GFP阳性细胞，表明GAD 67在生命早期表达。在这项研究中，我们提出了我们的研究结果在胃和肠GAD表达细胞的初步结果，并讨论GAD表达的意义和这些细胞的作用。
Expression of glutamate decarboxylase in mouse stomach and intestine was studied by RT-PCR and immunohistochemical techniques. The RT-PCR study showed GAD mRNA expression in fetus and postnatal ages of 4 and 11 weeks old mice. A strong positive staining against GAD65 was observed at both stomach and intestine of the 4- and 11-week old mice. GFP positive cells were found in the fetus at both stomach and intestine, suggesting that GAD67 is expressed early in life. In this study, we present preliminary results of our findings on GAD expressing cells in stomach and intestine and discuss the significance of GAD expression and role(s) of these cells.
DOI：
10.1073/pnas.94.25.14060
发表时间：
1997-12-09
影响因子：
11.1
作者：
Kash, SF;Johnson, RS;Baekkeskov, S
通讯作者：
Baekkeskov, S