THE DETERMINATION OF WATER IN ERYTHROMYCIN BY FISCHER,KARL TITRATION
THE DETERMINATION OF WATER IN ERYTHROMYCIN BY FISCHER,KARL TITRATION
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DOI：
10.1016/0731-7085(88)80013-x
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发表时间：
1988-01-01
影响因子：
3.4
通讯作者：
HOOGMARTENS, J
中科院分区：
文献类型：
--
作者：
CACHET, T;HOOGMARTENS, J
卡尔·费休法测定的红霉素样品的水分含量在实验室间发生了重要的变化。结果表明，这种变化与所用试剂的类型有关。在缓冲能力较差的体系中，红霉素的酸降解生成红霉素烯醇醚和水。然而，当使用适当的溶剂时，准确滴定红霉素中的水是可能的。10%m/v的咪唑甲醇溶液比吡啶或吡啶与甲醇的混合物有更好的缓冲能力，没有吡啶的难闻气味，滴定速度快。
Important interlaboratory variation was obtained for the water content of erythromycin samples as determined by the Karl Fischer method. It is demonstrated that the variation is related to the type of reagents used. In poorly buffered systems erythromycin enol ether and water are formed by acid degradation of erythromycin. However, when an appropriate solvent is used, accurate titration of water in erythromycin is possible. A 10% m/v solution of imidazole in methanol is preferred to pyridine or a mixture of pyridine and methanol for it has a good buffer capacity, it lacks the unpleasant odor of pyridine and it allows a high titration speed.