THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE POPP (1967) STRAIN OF MARBURG-VIRUS - A COMPARISON WITH THE MUSOKE (1980) STRAIN
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE POPP (1967) STRAIN OF MARBURG-VIRUS - A COMPARISON WITH THE MUSOKE (1980) STRAIN
复制标题
DOI：
10.1007/bf01322532
复制
发表时间：
1995-01-01
影响因子：
2.7
通讯作者：
NETESOV, SV
中科院分区：
文献类型：
--
作者：
BUKREYEV, AA;VOLCHKOV, VE;NETESOV, SV
测定了马尔堡病毒Popp株基因组RNA的核苷酸序列。Popp株于1967年在第一次丝状病毒爆发期间分离。病毒是从受感染的豚鼠血液中纯化出来的。测定的序列长度为19112个核苷酸。推导了七种已知病毒粒子蛋白的氨基酸序列。核苷酸和氨基酸序列与1980年在肯尼亚分离并从Vero细胞中纯化的马尔堡病毒Musoke株进行了比较。核苷酸序列同源性为93.9%。比较显示核苷酸和氨基酸序列的保守区和可变区。发现病毒粒子的包膜蛋白GP是最可变的蛋白质。蛋白质的最大差异位于分子的外部。L蛋白是病毒RNA依赖性RNA聚合酶的主要成分，其氨基酸替换也是极其非随机分布的。结果表明，基因组的非编码区比编码区的变异更大，37.6%的核苷酸差异对应于前者。72.6%的核苷酸替换发生在编码区的第三个密码子位置。
The nucleotide sequence of genomic RNA of Marburg virus strain Popp was determined. Strain Popp was isolated in 1967 during the first filoviral outbreak. The virus was purified from blood of infected guinea pigs in which it had been maintained. The length of the determined sequence was 19112 nucleotides. Amino acid sequences of seven known virion proteins were deduced. Nucleotide and amino acid sequences were compared with those of strain Musoke of Marburg virus isolated in 1980 in Kenya and purified from Vero cells. Homology between nucleotide sequences of two strains was 93.9%. Comparisons revealed conserved and variable regions of the nucleotide and amino acid sequences. The GP, the envelope protein of the virion, was found to be the most variable protein. The greatest differences in the protein were located in the supposedly external part of the molecule. Amino acid substitutions in the L protein, the main component of viral RNA-dependent RNA polymerase, were also distributed extremely non-randomly. It was shown that the non-coding regions of the genome were more variable than the coding ones; 37.6% of nucleotide differences corresponded to the former. 72.6% of nucleotide substitutions located in the coding regions were found to be at the third codon position.